Spectrum Analysis

Spectral flow cytometry is used to sample more parameters per single cell or particle than traditional flow cytometry. In contrast to conventional flow cytometers, spectral flow analyzers and sorters substitute mirrors, optical filters, and photomultiplier tubes (PMTs) with a spectrograph and multichannel detector to use fluorescence or Raman spectroscopy.

 

FCS Express 6 now introduces the capability to read raw spectral data from Sony instruments stored with the .SRAW file format and a new 1D plot type - Spectrum Plots.  To analyze the spectral data in FCS Express, .sraw and .fcs files must be in the same source folder.

 

Data can be presented in the spectrum plot with one spectrum line per event (Format→Plot Options→Overlays→Overlays to showAll Spectrums command) or one line as an average of events (Format→Plot Options→Overlays→Overlays to showAverage command). The color of each line will reflect the color of the gate in which the event falls if Overlay to show is set to All Spectrums.  If set to Average, the color will be derived from Line options color. See Formatting a Spectrum Plot for more information on these and other formatting options.