Spectrum Plots are a special subset of the one-dimensional plot type that displays spectra for all events within a gating strategy as individual lines or an average of all events within a gating strategy as one line. Spectral lines for cells or particles are displayed as Counts (Y-axis) vs. wavelength from 500 nm to 800 nm (X-axis) for the specified laser line. Compensations and/or transformations may be applied to spectra displayed.
Data can be presented in the spectrum plot with one spectrum line per event (Format→Plot Options→Overlays→Overlays to show→All Spectrums command) or one line as an average of events (Format→Plot Options→Overlays→Overlays to show→Average command). The color of each line will reflect the color of the gate in which the event falls if Overlay to show is set to All Spectrums. If set to Average, the color will be derived from Line options color. See Formatting a Spectrum Plot for more information on these and other formatting options.
To insert a Spectrum plot, first ensure that the data files meet the requirements for the data format. Then, use the Insert→1D Plots→Spectrum command (Figure 28.1).
Figure 28.1 - Insert Spectrum Plot command
An example of a Spectrum Plot is shown below in Figure 28.2.
Figure 28.2 - Inserted Spectrum Plot
Spectrum Plots can be formatted to show interpolated values, gate colors, averages, population density, and more.