## How do I display my data in FCS Express as it appears in the Summit Software?

FCS data in the Summit software is displayed on a 0-256 linear or 0-100,000 logarithmic scale. However, the data is stored in the data file as 32 bit data with a range of 0-4,294,967,295. When the data is viewed in FCS Express the 32 bit native values are displayed on the axis by default and used for subsequent calculations such as means and medians which may appear different than it originally looked in Summit Software. The instructions below may be followed if you are noticing a problem with the way the data is displayed.

### How to rescale Summit data (after 6.004.0034 release)

In the newest version of FCS Express, an option is available for users to scale fluorescence parameters on a 5 log decade scale so the data is displayed identically to Summit Software. To acess this feature, you will need to open your FCS Express User Options by selecting the **File** tab and selecting **FCS Express User Options** from the drop down menu. Once the FCS Express User Options window is available,** FCS File Options** can be selected fom the expanded view of the **Data Loading** option. The **Scale Summit fluorescence parameters **box can be checked. This will update the user preferences to scale Summit data in 5 log decades anytime a file is loaded. This preference change will only need to be done once for each user. Below is an example of the difference of the default scaling and the updated scaling.

FCS Express User Options |

Native Summit Data |
Newly Scaled Summit Data |

## How to rescale Summit data in older versions of FCS Express

There are two options for rescaling or displaying the data in FCS Express

- Use the
**Divide by Axis Labels**feature: Using the Divide by Axis Label feature will display the axis as they are seen in Summit but will not change the native raw data or statistical values. - Use the
**Parameter Math**Feature: Using Parameter Math will create a new parameter based on the scaling factor. The native raw data values will also be scaled accordingly.

### Using the Divide by Axis Labels Feature

The **Divide by Axis Labels** feature may be accessed by right clicking on a plot>choose **Format**>**Axis** Category>**Divide By Factor**. Using the Divide by Axis Labels feature will display the axis as they are seen in Summit but will not change the native raw data or statistical values.

For Linear Parameters type the value |

For Logarithmic (fluorescence) Parameters type the value |

Once values have been entered click **OK**. Below is an example of the data before and after making the Divide by Axis Labels change.

Summit data before applying the Divide By Axis labels change. | Summit Data after applying the Divide By Axis labels change. Notice the Axis values have been updated according to the scaling factor but the X and Y Median values have not changed. |

### Using the Parameter Math Feature

The **Parameter Math** feature may be accessed in the Transformation Navigator from the **Tools **tab>**Transformations**>**Transformations** command. More detailed information about setting up parameter math can be found in our Using Parameter Math Manual Topic. Using the Parameter Math feature will create new parameters based on the scaling factor resulting in parameters that are displayed on the correct scale and raw data values that reflect the scaling change.

In Transformations navigator, create new parameter math and click **New ** below **Formula Sequence** box to add sequence for linear parameter. The **Create New Sequence Item** dialog will appear. Enter formula as depicted in image below and click **OK**.

For Linear Parameters use the scaling factor of |

In Transformations navigator,click **New ** below **Formula Sequence** box to add new sequence for logarithmic parameter. The **Create New Sequence Item** dialog will appear. Enter formula as depicted in image below and click **OK**.

Once parameter math formula sequences have been defined, **click and drag** the newly created parameter math from the Transformation navigator to the plot. Below is an example of the data before and after performing parameter math on the data. Please note that you may also overwrite the original parameter by choosing it from the **Output Parameter** drop down in the **Create New Sequence Item **dialog. Once applied, the new parameters will be available from the x- and y- axis drop down lists on the plot, and "Parameter Math applied" will be appended to the plot title.

Summit data before applying performing parameter math on the data. | Summit Data after performing parameter math. Notice the Axis values have been updated according to the scaling factor and the X and Y Median values have changed to reflect the newly created parameters. |