FCS Express 4 Manual

Creating a Gate from a Marker

Creating a Gate from a Marker

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Creating a Gate from a Marker

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To create a Gate from a Marker on a 1D plot:

1.Press the Page 2 tab from the Layout Navigation Toolbar (lower left of the window). You will see a blank histogram and dot plot on that page.
2.Select the Home→Editing→Select All command to select all plots.
3.Select the Data→Change Data on Selection→Select command.
4.Select the file ApoMono.002 located in the Tutorial Sample Data archive.
5.Press Open on the file dialog to accept the file choice.
6.Click and hold down the mouse button on the X-axis label, (FSC-H), of the histogram.
7.Scroll the mouse to choose FL2-H as the new X-axis label.

The histogram should now look similar to Figure T4.11.

 

Figure T4.11  Histogram with Marker

Figure T4.11  Histogram with Marker

 

8.Right-click on the histogram to bring up the pop-up menu.
9.Select Convert Marker to Gate→Marker #1 (M1) →Convert and Link from the pop-up menu (Figure T4.12).

 

Figure T4.12  Convert Marker to Gate

Figure T4.12  Convert Marker to Gate

 

A dialog will appear asking you to name the gate.

10. Enter "Marker Gate" in the Create a new gate named edit field.
11. Press OK to accept the name.

The gate is now linked to the marker because we selected Convert and link above. This means that whenever we move the marker, the gate will also move to reflect the new marker position.

 

In the next section, we will create a gate from a quadrant.