knowlegebase: Adjusting Density plots saved in FCS Express v3 for use in FCS Express v4

FCS Express V4 includes many improvements in density and contour plotting, including the ability to view outliers, new color schemes, and color level options. Because of these improvements some density plots that were saved in version 3 may not appear exactly the same in version 4. Notice in the images below that a density plot was first saved in version 3 (left) and opened in version 4 (center). Some events are now being treated as outliers in version 4 and they appear in black (center top) while other times no outliers appear at all (center bottom). By ensuring outliers are enabled and by adjusting the outlier color to be red the density plot appears can look identifdal in version 3 and 4. Please follow the steps below to adjust density plots saved in version 3 so that they appear the same in version 4.

Density Plot Saved in FCS Express version 3.
Density plot saved in FCS Express version 3 and opened in version 4. Appears with outliers (top) appears without outliers (bottom).
Density plot saved in FCS Express version 3 and adjusted for display in version 4.

Steps for correcting density display issues:

To correct one plot at a time

To correct multiple plots at once
  1. Right click on the plot you would like to adjust.
  2. Choose Format in the drop down list.
  3. Choose the Overlays category.
  4. Ensure the Show Outliers box is checked.
  5. Change the Outlier Dot Color to Red.
  6. Click OK.
  1. Select all of the density plots you would like to adjust.
  2. Select the Format tab>Plot Options>Overlays.
  3. Ensure the Show Outliers box is checked.
  4. Change the Outlier Dot Color to Red.
  5. Click OK.