FCS EXPRESS: Gates

In FCS Express, to analyze cell sub populations, you create gates and apply those gates using one of many methods.  FCS Express supports five types of drawing gates, and another type, called combination gates, which can  combine any number of other gates in several ways. You can create an unlimited numbers of gates. Our video on gates will show you how easy and powerful they are to use.

Rectangular Gates:
Gates that are in a square shape.		 Elliptical Gates:
Gates that are in an elliptical shape		 Polygonal Gates:
Gates that are in the shape of a polygon which are defined by several vertices and straight lines connecting the vertices.

Freeform Gates:
Gates that are drawn in an arbitrary shape. The gate boundaries can be curved.
1D Gates:
Gates that exist only in one dimension.		 Well Gating on Heat Maps*: Gates that are applied to one or many wells on a heat map plot.

* only available with high content features

Applying A Gate

FCS Express makes it easy to to apply a gate (ie. restrict the cells which appear on a plot to the cells that pass through a specific gate).  View the short videos below for exampes of:

  • Applying a gate from the gating toolbar
  • Dragging and dropping a gate to create a new plot
  • Dragging gates from the gate navigator to a plot

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Applying a gate using the gating toolbar

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Dragging and Dropping Gates

Hierarchical Gating

FCS Express supports hierarchical gating to create gates based on subsets of other gates. In the example below a "Live Cell" gate was created with subgates created for Fl-1 and FL-2 positive cells. A further hierarchy was created for those cells in Fl-1 that are dual positive.


FLEXIBLE GATING FORMULAS

For more sophisticated gating, you need to combine more than one gate into a formula. To create the formula, simply open a Gate View window, right-click, and select Add Combination gate.  You will then be able to use boolean expressions to create powerful new gates.  Below is an example of using combination gates to include cells in Gate 1 and Gate 2, Gate 1 or in Gate 2, and lastly excluding those cells in Gate 1 and Gate 2.
Right clicking on the Gate View window will allow you to Add a Combination Gate.