If you have any questions which do not appear on this list, please do not hesitate to contact us directly.

  1. What operating systems does FCS Express run on?
  2. What is your refund policy?
  3. What is a configuration file?
  4. What is a countercode?
  5. What is the difference between the demo, lite and professional versions?
  6. I have saved my layout as a PowerPoint presentation. Can I edit the text in the text boxes or on the plots?
  7. Why are there access violations when I save and load files?
  8. Why does the text on the right-most x-axis label cut off on my plot?
  9. How does FCS Express implement software compensation?
  10. How can I track a population in FCS Express?
  11. Why do I get errors upon exiting FCS Express?
  12. My computer which was running FCS Express had to be replaced. How do I reinstall FCS Express on my new machine?
  13. Why does FCS Express crash immediately upon starting up?
  14. Where is the configuration information for FCS Express stored?
  15. How can I set up FCS Express to read data saved by an Abbott CellDyne Sapphire?
  16. How can I set up FCS Express to read data saved by an Accuri C6?
  17. What are the Resolution Options on the General Category of the plot formatting screen?
  18. Why do my dot plots appear sparse and/or blocky?
  19. Why does the compensation on my Coulter FC 500 data seem incorrect?

Why does my compensation on FC 500 data seem incorrect

Why does compensation from my FC 500 listmode files seem incorrect?


Coulter listmode data files really contain two datasets inside. The first data set is 10 bit FCS 2.0 data. This is the data set you are used to seeing. The second data set is 20 bit FCS 3.0 data.

Typically, users use the first data set, although FCS Express can load the second if the user wishes. The first data set is saved already compensated, there is no way to uncompensate it. The first data set also contains the compensation matrix that was used with the first data set.

FCS Express has several options regarding what to when it encounters a data file that has a compensation matrix embedded. This can be found in File->Edit Preferences on the General category as Default Compensation To Use. If you are using the first data set of the Coulter file, set this value to none. If this is not set to none, you are telling FCS Express to apply the compensation matrix to a data file that is already compensated, causing overcompensation. 		 
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