De Novo Software

Continuing Education - FCS Express Clinical Webinar Series De Novo Software is pleased to announce that we have been approved as a provider of continuing educational programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program.

We are pleased to announce our first webinar to offer continuing education for our customers.

Clinical Flow Cytometry Data Analysis Case Examples

The purpose of this webinar is to provide participants with a means of improving their current flow cytometry data analysis through examples provided by other clinical flow laboratories.
Three example analysis templates will be presented by the labs the created and use them in their daily workflow.

Each lab will present their rationale behind the marker selection and gating strategies used, their incorporation of quality checks and alerts, and the ways in which they have tried to standardize and provide accurate patient results. There will be plenty of time at the end of the webinar to ask the speakers any questions that you may have. This is a great opportunity for you to learn and share with other flow labs!

Intended Audience: Medical technologists, Medical Laboratory Technicians, Clinical laboratory professionals engaged in performing flow cytometric assays, pathologists and pathology residents.
Level of Instruction: Intermediate
Contact Hours Provided: 1.0 hour

Speakers and Cases to be presented:

Eddie Hutchison MPH, MT (ASCP) Manager of Immunopathology St. Jude's Children's Hospital Memphis, TN 38105 Case topic: Lymphocyte subsets
Dan Marmer M.S., MT (ASCP) Manager Core Flow Cytometry Facility Cincinnati Children's Hospital Medical Center Division of Hematology/Oncology Cincinnati, OH 45229 Case topic: CD34 Analysis
Fred Daugherty, MT (ASCP) Laboratory Manager Flow Cytometry and IHC CSI Laboratories Alpharetta, GA 30005 Case topic: Hairy Cell Leukemia
Tanya Tolmachoff, MT (ASCP)SI De Novo Software Los Angeles, CA 90010 Webinar Introductions and Moderator

Educational Objectives: Upon completion of this activity, participants should be able to:

1. Diagram where to find the different cell populations in a FSC vs. SCC plot.
2. List which populations are marked by these commonly used CD Markers: CD3, CD4, CD8, CD19 and CD16/CD56.
3. Perform a lymphosum calculation and describe why this QC check is used for subset analysis.
4. Describe the components required to calculate age-specific normal range comparisons for reporting patient results.
5. Summarize the "ISHAGE" gating strategy for detection of hematopoietic CD34+ cells, and the exclusion of non-viable cells by dye uptake.
6. Determine the presence of CD3+ and CD34+CD19+ cells populations in a specimen.
7. Examine a final report with formulated tokens that calculate and display quantitative data.
8. Describe the classic morphology associated with Hairy Cell Leukemia (HCL).
9. List the commonly used CD Markers to detect HCL.
10. Discuss the utility of CD123 in the detection of HCL.

If you wish to receive the contact hours awarded for this program, you will need to register and log on individually for the webinar. Afterwards, you will need to complete the program evaluation survey to obtain your Certificate of Attendance. The link to fill out the program evaluation survey will be emailed to you when the webinar is completed.

Note: If multiple people at your facility would like to view the webinar together, please contact us to receive the attendance verification roster and instructions for completing the program evaluation survey.

document.write(titletext);

Clinical Flow Cytometry Data Analysis Case Examples