FCS Express: Tips for Using BD Accuri C6 Software data in FCS Express

The BD Accuri C6 is a bench-top system that delivers performance and features similar to those of the market-leading flow cytometers, yet it is easy even for novice users to set up and operate. The BD Accuri C6 Software that controls the C6 is easy to use and allows even a novice to quickly set up an experiment and acquire data.

There are two ways to load data from BD Accuri C6 Software files into FCS Express:

  1. By using the BD Accuri C6 Software importer, which brings in the set of plots and gates that were defined in the BD Accuri C6 Software, as well as the data itself.
  2. To simply open a plot in FCS Express, and then when a dialog appears asking you for a data file, choose a BD Accuri C6 Software file (with extension c6). There are a few things to note about loading data files this way:

Recommended changes to FCS Express User Options

  1. Open the preferences by selecting the File tab-> Options.
  2. Select the category Data Loading->FCS File Options. Turn off "Allow Multiple Data sets". Note that this change is not necessary for loading a BD Accuri C6 Software data file, but will allow you to add the subfiles to the file list, which is necessary to do batch processing.
  3. Turn off "Validate that files are FCS Files before displaying them in a file dialog". Note that this change is not necessary for loading a BD Accuri C6 Software data file, but will speed up adding files into the file list.

BD Accuri Preferences

Figure 1 - Recommended Data Loading Options for BD Accuri C6 Software data in FCS Express

  1. Select the category Dialog->Open Data Dialog. Check the Advanced Data Dialog checkbox. Note that this change is necessary for loading a BD Accuri C6 Software data file as single files. To open the data set as a plate, the standard data dialog is sufficient.

Figure 2 - Recommended Dialog Options for BD Accuri C6 Software data in FCS Express

Loading the data into a plot

1. Proceed to open a plot, just as you would with any other data file. When the Open Data File dialog opens, click on Select data file.

2. In the Select data file dialog change the file type to be BD Accuri C6 files (if the files within the C6 file need to be loaded as single files) or BD Accuri C6 Plate files (if the C6 file needs to be loaded as plate).

Figure 3 - BD Accuri C6 Software file type in the file dialog

3. Navigate to the BD Accuri C6 Software file that you would like to open and double-click on it.

  • If BD Accuri C6 files has been selected as the File of type, the Open Data File dialog will then display all of the files contained inside the BD Accuri C6 Software file. Select the file you would like to load (see Figure 4).
  • If BD Accuri C6 Plate files has been selected as File of type, the Open Data File dialog will then display the BD Accuri C6 Software file. Select it.

 

    4. Click Open. A plot will be created displaying the selected file.

 

Data List considerations

  • When you open a standard .FCS data file within a plot in FCS Express the software will load every data file from the same directory into the Data List. However, when you load a data file from within a BD Accuri C6 Software file, the Data List will not be automatically populated. However, you can easily populate it yourself before or after inserting a plot via the instructions above.
  • To quickly load multiple data files into the Data List, open the Data List from the Data tab -> Data List. Press the Add button. Select BD Accuri C6 files as Files of type, navigate to the BD Accuri C6 Software file that you want to open, and double-click on it. The dialog will then display all of the files contained inside the BD Accuri C6 Software file. Use Ctrl+Click or Shift+Click to select all files you would like to add to the data list and click Open.
  • Once the Data List is populated with the BD Accuri C6 Software data, you can perform all of the normal operations on the plots that you would with other data files - for example, using the Next and Previous buttons, and batch processing, or changing the files directly from the Data List.

Figure 4 - Selecting several data files from inside the BD Accuri C6 Software file

Using Multicycle with BD Accuri C6 Software Data

FCS Express and Multicycle are fully compatible with you BD Accuri C6 Software data. To get tips and learn more about using multicycle with your BD Accuri C6 Software Data please see our Using Multicycle with Accuri Data knowledge base page.