What's New in FCS Express 6

FCS Express 6 brings with it Mac and PC compatibility, more speed, cluster analysis, R-integration, innovative integrated spreadsheets, Amnis data file compatibility, Sony spectral file compatibility and much more.

With FCS Express 6 you will be spending less time on your analysis and more time doing what you do best. Check out the full list of our new features below and make sure to download a free FCS Express 6 demo to give it a try today.

Mac and PC Compatibility

FCS Express 6 is now compatible with any Mac or PC computer. Features in both operating systems behave the same way and any license may be used on either system. Please see our system requirements page and downloads page for more information and to get started today.

Data Specific Gates

Data Specific Gates allow you the ability to assign gates to specific data files that have been loaded in the data list. Different gating positions between data files may be stored and automatically used when displaying plots or making calculations. Learn more about using the Data Specific Gate features.

Data Transformations, Cluster Analysis and R-Integration

Data Transformations allow you to create custom parameters for k-means clustering, principal component analysis, SPADE, tSNE/viSNE as well as allow you to directly integrate R-scripts. The new transformations tools are built directly into FCS Express with an easy to use interface and drag and drop to plots capability. Learn more about using the Data Transformation features.

Integrated Spreadsheets

Spreadsheets can be used to perform most of the operations that Microsoft Excel provides. In addition, values in Spreadsheet cells can be derived directly from statistics within the layout, keywords, or from data files in the data list. Insert statistics for data files in the data list allows additional flexibility in analysis of many statistics for many data files that are not currently open in the layout. Learn more about using Spreadsheets.

Marker to Marker Linking

FCS Express 6 allows users the ability to link any markers together so that moving a marker end point will automatically cause an end point on a different marker to update at the same time. This allows for live updating bifurcation of markers on histograms. Linking markers is accomplished by setting the low or high bound for one marker to use a token value for the high or low bound of a separate marker. Learn more on our markers resource page.

Standard Operating Procedures

The Standard Operating Procedure feature allows you to create a predetermined set of steps in your FCS Express layouts in which each step can be conditionally formatted with tokens so that a requirement must be met before the next step is performed. For instance, a user may not proceed to the next step unless the number of events in a gate is above a certain threshold value or text has been entered in a field. There are a wide variety of applications for SOP's and we encourage you to learn more at our SOP resources page.

Permission Rules and Authorizations

All objects in FCS Express 6 may now be assigned Authorizations and Permission Rules, or rights, which allows or prevents layout users from performing certain actions. Permissions can be used to allow or prevent any action while Authorizations may be set to allow or deny placing objects in Select mode or Edit mode or both. Authorizations can be set to always allow, never allow, or always allow/do not allow based on the value of a token. For instance, a token value can be set to allow selection of a plot if the current FCS Express user equals a specific person or a plot can remain non-editable unless a particular statistic value is met, ie the number of events for a particular gate is higher than X. Learn more about Authorizations and Permission Rules.

Locking and Visibility Options

Visibility of any objects or pages may be set as visible, not visible (hidden) or conditionally based on a token value. Conditional visibility has many potential applications and is especially useful when creating Standard Operating Procedures when an objects visibility may be based on the current step in the SOP.

Additionally, marker, quadrant, and gate editing can be set to allow, not allow, or allow based on a token to "lock" down movement of these items. Markers and Quadrants share the same visibility features mentioned above for objects.

Learn more about Visibility and Locking.

General Usability

The team at De Novo Software is dedicated to making otherwise complex experiments easy to analyze in FCS Express. Below is a partial list of some features that have been added to improve your experience:

Please see the FCS Express 6 release notes and the FCS Express Manual for a detailed list of new features

Amnis .DAF file Image Cytometry Compatibility

The team at De Novo Software is exited to announce direct compatibility with .DAF files from Amnis IDEAS software. The teams at Amnis and De Novo Software have worked closely together to bring all of the advanced reporting capabilities FCS Express Image cytometry offers to your advanced image cytometry experiments from IDEAS software. Simply save your .DAF and .CIF files from IDEAS and open them in FCS Express. Images will be loaded directly in Data Grids within FCS Express and will update in real time as gates change. Learn more about compatibility on our Amnis Knowledgebase page.

Sony Spectral Data Compatibility

FCS Express 6 now introduces the capability to read raw spectral data from Sony instruments stored with the .SRAW file format and a new 1D plot type - Spectrum Plots for analysis the spectral data. Data can be presented in the spectrum plot with one spectrum line per event or one line as an average of events. The color of each line will reflect the color of the gate in which the event falls effectively providing backgating information for spectral data. More plot visualizations and features will be coming soon. Learn more about Spectrum plots and analysis.

Heat Map Overlays and Radius Displays

FCS Express 6 introduces the capability to display wells with a radius parameter that will adjust the radius of wells in real time as the data changes effectively allowing you to visualize additional parameters without trade off. For instance, one could plot the median of a parameter of interest as the heat parameter and the number of events as the well radius. Additionally, heat maps now support up to six overlays which are represented as slices of a "pie" within the well. Combining overlays with radius based displays will allow for easy visualization of many parameters. Learn more about heat map overlays and radius displays.