This page allows you to download the latest release of FCS Express Version 3 Research Edition. To upgrade the Clinical Edition click here.

If this is your first time downloading FCS Express, please go here instead.

The current release of FCS Express is 3.00.0612. You can check your release number by selecting Help->About.

Click on the link below to download the upgrade. Once you have downloaded the upgrade executable file, simply run the file, and it will begin the upgrade process.

Changes for Version 3.00.0612 (August 12,2008)

Enhancements

• Can now specify Name+Stain or Stain+Name for the parameter labels on plots
• Added support for data files from latest version of Guava software

Bug Fixes

• Improved handling of Multicycle errors, and fixed a problem in Multicycle integration which will prevent crashes
• Fixed a problem that caused a crash on startup for network uses, where Multicycle was installed but the user did not have admin rights
• Fixed the high bound statistic on histograms with no markers with FCS 2 data files
• Fixed a crash that occured if you had two tokens side-by-side in a table cell, and then undid creating the second token
• Fixed a problem in the CFlow importer where not all files in the analysis tab were imported
• Make sure that the dongle drivers are not installed for a second user on the computer, if they've already been installed under a different user
• Updated several tutorials with newer screenshots of FCS Express

Changes for Version 3.00.0611 (May 23,2008)

Enhancements

• Gates now appear alphabetically ordered in drop down list on gating toolbar

Bug Fixes

• Improved speed when browsing FCS files on a network drive.
• Speed improvements in Accuri Importer
• Statistics windows were not opening in the correct position on multi-monitor systems
• Quad stats were not always correct when importing Accuri data
• Network multicycle required admin right to install on the local computer
• Gates would occasionally dissapear if you double click in them

Changes for Version 3.00.0607 (April 10, 2008)

Enhancements

• Added the Proliferation module
• Added Accuri CFlow file import
• Updated the Multicycle integration so that network installations of FCS Express can use Multicycle without dongles

Bug Fixes

• Fixed a crash when trying to show compensated data in the data form
• Exporting the first data set of a multi-dataset file would not clear the $NextData keyword
• Transition points of hyperlog, lin/log could not go over 999
• TaskBar did not show a scrollbar if it was longer than the screen
• Compensation was not being read properly for the 2nd data set in coulter files
• Fixed some minor problems importing Diva layouts
• S-phase token did not recalculate when updating DNA plot
• If the dongle was removed during analysis, too many warning messages would appear
• Undoing a marker create and then a marker delete in a specific sequence would cause a crash
• Even if Multicycle histogram plot had no data, it still attempted to perform a Multicycle DNA fit
• if quads were linked on plots that had different axis minima, the quad lines did not go to the bottom
• In rare occasions when resizing gates, a vertex that was not being resized would move by one pixel
• Marker stats did not work correctly when applied to overton overlays
• Custom tokens containing tokens referring to data files on disk, rather than data files in plots, caused problems
• Minor fixes related to the Multicycle DNA module
• Quad stats for 2D plots previously did not use the concentration settings
• Calibration on plots with negative populates previously caused crashes
• The gate drop-down list in the gating toolbar was previously limited to 8 gates

Changes for Version 3.00.0601 (January 2, 2008)

Bug Fixes

• Fixed problems with displaying buttons on several windows when the display settings of the monitor were set to use large fonts.
• Previously, compensation matrices from data files generated by BD Canto clinical software were not loaded correctly.
• Previously, the file list window did not correctly use the setting for "Display Full Path".
• Eliminated a crash that occasionally occurred when trying to load a file, and clicking on a shortcut to a network path inside the open file dialog.
• Fixed problems that could cause gates to get cut off at the value 1000.
• Fixed a problem that could cause gate points to change when they were against an axis.
• In the previous version, saving PDF file in batch processing did not respect the landscape vs portrait settings.

Changes for Version 3.00.0504 (October 22, 2007)

Bug Fixes

• Fixed a problem in the security system that could cause the databases to lose user information.
• Fixed a problem where the configuration information changed when upgrading the clinical edition to a newer release.
• Fixed a problem related to loading a new set of data files into a layout with a panel, where the layout previously had more files.

Changes for Version 3.00.0503 (October 8, 2007)

Enhancements

• Windows Vista support.
• Added support for compensation in Diva 6-generated data files.
• Sped up loading and closing layouts.
• The installation program and program executables are now digitally signed.
• Added support for Word 2007 when exporting Word document reports.

Bug Fixes

• Fixed a crash in Chinese systems when loading any UI elements that needed passwords.
• Previously, putting a background image in plots could cause the layout to grow larger and larger.
• Fixed an issue with database corruption when multiple people logged on as the same user, in the security system.
• Fixed a crash that was caused by pasting text boxes with stats from a plot onto a blank part of the layout.
• In the previous version, the quad lines did not extend down to the axes in scalings with values < 0.
• Fixed a crash that could occur if you opened more than one plot at a time using FCS File -> Open.
• Fixed a problem where marker gates on imported Diva experiments might be offset.
• Previously, undoing a gate delete sometimes led to a crash if there was a stats window open.
• Minor fixes to permissions in advanced publishing options.

Changes for Version 3.00.0415 (August 22, 2007)

Bug Fixes

• Minor fixes to permissions in advanced publishing options.

Changes for Version 3.00.0413 (August 2, 2007)

Bug Fixes

• In release 412, if you had upgraded from a previous release and then published a layout, the resulting published layout did not give people permissions to perform any actions, such as moving gates.  This has been fixed. 
• Fixed a problem that parameter math did not always set up the new parameter's scale information correctly. 
• In release 412, if there were parentheses in the file name, that file name did not appear in the file open dialog. 
• Fixed a crash that occurred when double-clicking on a data file, and trying to open it with FCS Express. 
• Improved the default worksheet names when running batch processsing with Excel Cell Mode actions.

Changes for Version 3.00.0412 (July 25, 2007)

Bug Fixes

• Fixed issues relating to the default data set option.
• Now handle large data files (> 100 MB) better.
• Fixed an issue with decimal separators in custom tokens.
• Fixed a problem with gates being clipped when the plots are in hyperlog scale.
• Improved memory requirements when saving layout files with linked data files.
• Fixed a bug where the quad vertices were in the wrong place after copying and pasting a plot.
• Fixed issues relating to drawing plots when alpha blending is enabled.
• Eliminated crashes related to having very small plots (1 x 1 pixels)
• Fixed a problem that could cause crashes when calculating Channel Calibration

Enhancements

• Introduced Publishing, and the FCS Express Reader.
• Added GetElapsedTime function in custom tokens.
• Separated axis options for FCS 2.0 and FCS 3.0 files.

Changes for Version 3.00.0320 (May 24, 2007)

Bug Fixes

• In version 3.00.0319, layouts saved with data set links to compound data files did not reload the data when they were reloaded.

Enhancements

• Improved speed in plotting 2D high-resolution data.

Changes for Version 3.00.0319 (April 24, 2007)

Bug Fixes

• In version 3.00.0316, text boxes inside plots were not always printed in the correct aspect ratio.

Enhancements

• Improved speed in four areas: loading layouts, populating a layout with data files, batch processing, and closing layouts.

Changes for Version 3.00.0316 (February 26, 2007)

Bug Fixes

• In the previous version, text boxes inside plots were wrapping so that the last word was placed on a second line.
• Fixed a problem with markers linked to gates, that sometimes caused a crash.
• Other internal stability fixes.

Changes for Version 3.00.0315 (February 12, 2007)

Bug Fixes

• In the previous version, text boxes inside plots were not printed properly unless they were on the page that was showing.
• Fixed a crash that occurred when removing an overlay from a histogram, if there were also Overton overlays on the histogram.
• Prevented a crash from occurring when loading a data file with invalid compensation settings.
• Fixed a problem that happened when copying objects inside a plot to the clipboard.
• Fixed a problem copying and pasting quadrants.
• Fixed a crash that occurred after removing an overlay and pressing Preview in the formatting window.
• In the previous version, gates did not always include events that occurred against the top or right axes.
• Fixed two minor problems with importing BD FACSDiva experiment files.
• In the previous version, the channel calibration statistics on markers did not apply calibration to the markers.
• Other internal stability fixes.

Changes for Version 3.00.0314 (January 29, 2007)

Enhancements

• Sped up saving layouts with embedded data files.
• Added functionality to correctly handle the picket-fencing effect in high-resolution data files.
• Now allowing the only overlay on a plot to become invisible.
• Now checking the $DISPLAY keyword, present in Diva files, to determine how to display axes. >
• Added a setting for "default data set" in compound files.
• When reading the second data set in a Coulter FC500 data file, the keywords from the first dataset are also imported.
• Added an option for the axis lines to be displayed at the border of the plot or always at the zero value.
• Sped up loading of Diva experiments.

Bug Fixes

• Fixed the layout saving process so that the password policy xml file is not being written out.
• Prevented a crash when changing the axis scaling through File -> Edit Preferences.
• Drag and dropping stats columns to the batch processing window now automatically creates column headers.
• Previously, a bug prevented setting the histogram axis maximums correctly.
• Fixed a problem copying and pasting quadrants.
• Previously, there was a crash when you deleted multiple actions from the batch processing window.
• Fixed the "rebuild databases" function in the security system.
• In the concentration calculator window, you can now enter an arbitrary time.
• Previously, when loading a layout which was calculating concentration, and the data files could not be found, it crashed.
• Other internal stability fixes.
• Fixed a problem reading in compensation in Coulter-generated data files that caused a crash on non-English systems.
• Previously, the "Default compensation" setting was not being saved.
• Fixed a problem with enabling and disabling the toolbar formatting buttons.
• Fixed a problem with copying stats to the clipboard.
• Resolved a crash involving changes to the resolution options on the General formatting category.
• Fixed a problem with setting the autoscale option in Axis formatting.
• Previously, gates drawn on the top or right wall of a plot did not include the events along the wall if the plots resolution was less than 512x512

Changes for Version 3.00.0311 (October 12, 2006)

Enhancements

• Enabled FCS Express to display DNA plots using Multicycle.
• Can now merge different FCS files together, using Tools -> Batch Export
• Added features for CFR Part 11 Compliance.
• You can now multi-select plots and use the Format Quads tool even if they plots have different scales.
• Can now go through the automatic compensation settings without needing to use the entire wizard.
• Sped up the statistic calculations.
• Enhanced compensation reading so that compensation is now read from Diva 4.1 and 5.0 files, as well as 4.0
• Implemented new data transformations: Hyperlog, Log with Negative, and Lin/Log. To change your axes to use them, go to the Axes formatting category.
• When moving Quads holding down ALT or CTRL will allow them to move only in a single direction.
• Added Multiple Duplicate and Next/Prev
• Automatic Compensation Wizard settings are now saved with the compensation
• Automatic Compensation Wizard single stained controls can now be obtained from a plot or a data file

Bug Fixes

• Previously, when you dragged and dropped stats from the histogram stats grid to batch processing, the marker number was lost
• Insert Text Box from the Insert menu did not work
• Previously, quad statistics did not update if you Duplicate + Next on a plot with quadrants
• Histogram Subtraction (percent positive) did not take into account compensation
• Fixed bug saving layouts with embedded Guava files
• If there were no cells in a quad, the quad stat reported the wrong result
• new parameters in parameter math were not created with the correct scale
• Fixed a bug where changing the visibility of the X or Y axis did not work.
• Previously, linked marker gates in Diva experiments were not being imported correctly.
• Fixed a rare problem in validating alias gate names.
• Fixed a problem that could lead to polygon gates losing their first point after being moved on the plot.
• Stats tokens previously displayed a problem after being dragged from text boxes to Batch Processing Actions.
• Fixed a bug where changing the visibility of the X or Y axis did not work.
• Previously, linked marker gates in Diva experiments were not being imported correctly.
• Fixed a rare problem in validating alias gate names.
• Fixed a problem that could lead to polygon gates losing their first point after being moved on the plot.
• Stats tokens previously displayed a problem after being dragged from text boxes to Batch Processing Actions.
• Fixed a bug where the page templates did not work properly at 200% zoom.
• Fixed a bug where the if text boxes were on the master page, and the layout was not at 100% zoom, the text boxes would appear to be the wrong size.
• Previously, when performing duplicate and next on a plot when there was a gate selected, the gate was be duplicated instead of the plot.
• Improved the system for determining names for gate children when copying and pasting hierarchical gates.
• Previously, the Automatic check box did not become unchecked when typing in the axis range.
• Fixed bug that led to a crash when trying to set up the configuration for certain Diva xml imports.
• Fixed a problem that caused some text boxes with tokens to become larger after the layout was reloaded.
• Previously, duplicating a plot and its stats window at the same time led to some drawing problems.
• Now allow uncorrelated histograms to be gated on marker gates.
• Fixed a problem that led to unexpected ungated points appearing in color dot plots.
• Previously, the legend on histograms did not reflect the line style of the overlays - eg dotted, dashed, etc.
• Fixed a problem that led to statistics grids not being printed properly, if they hadn't been viewed yet in the layout.
• Fixed a bug when pasting a gate from one layout to another that happened if that gate had children
• Fixed a memory leak in Statistics Classifiers.
• Fixed a bug when copying a plot with quads from one layout to another.
• Fixed a problem with undoing pasting a marker.

Changes for Version 3.00.0209 (June 22, 2006)

Bug Fixes

• Fixed a problem that potentially occurred when changing plot settings which increased the size of histogram peaks.
• Fixed a problem related to lowering the resolution on histograms and dot plots.
• Fixed a problem that occurred when performing automatic compensation, if the gate selected for the single-stained control was empty.

Changes for Version 3.00.0208 (June 15, 2006)

Enhancements

• Keyword selection dialog now has selections outlined in highlight color, rather than just an arrow pointing to the selected keyword
• When gates are pasted, they can now replace another gate.
• Added a new option, which if turned on, will require holding down the alt key to change parameters with the mouse
• Added new option to allow the user to remove the frame around the plots.
• Made the batch processing window smaller for easier use on computers with 1024 x 768 resolution.
• Changed the automatic compensation wizard to use the file dialog which can read multiple data sets in a file.
• Introduced new, alpha-blended selection rectangles.
• Added the ability to display the current gate in a plot's title.

Bug Fixes

• Improved handling of errors which occur when the computer has run out of memory.
• Printing now does not should the gray outline of tokens, if the text box the tokens were in is in edit mode
• Fixed a problem that removing overlays did not remove all of the selected overlays
• Undoing the a change to Editing Preferences previously did not trigger the layout to redraw itself.
• Fixed a reference-counting problem with FCS Files when using the FCS File -> Open dialog.
• Fixed a problem in batch processing with exporting to Excel that occurs when the installed version of Excel is in a different language from the default language of Windows.
• Copying text to clipboard did not work for pasting into Word.
• Previous, it did not import DIVA 4.1 compensation parameters correctly.
• Fixed button order on Insert Picture Dialog
• Concentration calculation tokens were not updating when concentration calculation changed
• Entering gate comment attached the comment to all gates
• Could not scroll in Tools->Gate Info using the keyboard
• Overlay gate token did not always update when gate was changed on the plot
• fixed problems with DNA histograms when changing resolutions
• Loading an existing channel calibration did not enable the OK button.
• Icon in legend was not correct for overlays with non-solid fill patterns
• The open histogram form crashed when deleting all files
• Show full path checkbox was not remembering its state
• Inserting a compensation matrix into a textbox if the compensation matrix had no elements caused a crash
• Edit->Delete was not enabled properly if a gate was selected
• Bug in undoing a Z-order change
• Marker tokens were not displayed correct stat if the marker token also referred to a gate that was not the same as the one on the plot.
• Gate name textbox was not in the gate color.
• Linked/automatic quads would crash if there were not cells on the linked plot
• Stats windows did not remember that they were centered after they were loaded
• Placeholders were disappearing on non-visible pages
• Filename in stats windows did not obey the "show full path" setting
• Manual Compensation window did not allow you to manually enter values greater than 100
• Entering a space as the zoom value caused a crash
• Non-solid lines printed too skinny to the printer and PDF
• Copy As picture did not display proper background when "embed plot data as bitmap" was selected.
• Fixed the fonts on the data windows.
• Fixed a problem with displaying uncorrelated histograms.
• Fixed a problem that prevented making the y axis scale larger than the resolution.
• Fixed a problem that caused histogram subtraction to take a long time on files with high resolution, such as files generated from BD FacsDiva.
• Fixed a problem that sometimes led to a crash when moving a gate.
• Previously, using the arrow keys to change the dot size on 2D plots caused a crash.
• Previously, deleting an Overton overlay caused a crash.
• Previously, program sometimes crashed when reading in a badly-formatted FCS file header.
• Fixed a problem that could cause a crash in histogram subtraction.

• When more than one plot is selected, if you select Edit->Copy as Picture, then all selected objects will be included in the picture.
• Added an option for disabling the hints when hovering the mouse over plots.
• Added an option to not display text boxes with statistics when showing quadrants on a plot.
• Added an option to not display a text box with the gate name when creating a gate.
• Added an menu item under File to save the current selection as a picture.
• Text boxes from Diva are now imported.

• The list of parameters that pops up on a plot is no longer cut off by the boundaries of the plot.
• Previously, the font toolbar did not work properly for text boxes embedded inside plots.
• Statistics windows became distorted if they had the autosize property set.  
• Previously, if a line or arrow was perfectly horizontal, using the format screen to change its size could cause a crash. 
• Fixed bugs related to creating and renaming parameter labels.
• If the option to embed plot data as a bitmap was on, and the plot had a non-standard background, then the plot was not printed properly.
• While formatting titles for more than one plot at a time, if the Keyword filter list was defined, then this filter was not applied.
• Layout saving was improved to better handle errors that occur when saving the file.
• Fixed positioning of axis titles to prevent them from overwriting the labels.
• Fixed a problem loading a panel, so that it does not crash if a non-panel information file was selected.
• Previously, if a the layout was saved to a PDF file which was read-only, the program crashed.
• Eliminated toolbar flickering when changing layouts.
• Quads were not always imported properly from Diva.
• When adding overlays you were not allowed to multiselect in order to apply a gate to multiple overlays at once
• If you were on the file list tab of the file dialog and pressed OK without selecting a file, there was a crash
• After loading channel calibration from a file, previously the OK button could not be pressed

• When formatting several plots to change their titles, you can now select a keyword from the files themselves instead of the generic list. 
• You can now select a color when creating a new gate.
• Can now choose files from the file list when changing files from a file dialog
• Sped up printing during batch processing.  
• Added "embed plot data as bitmap" option when creating metafiles. This allows PDF files to be smaller and improves how plots look in MS Powerpoint.

• The master page now properly handles page number tokens. 
• Using tokens linked to data files is now implemented fully.  
• Text boxes embedded on plots were not displayed correctly when saving to a picture.  
• Improved the importing of text boxes from version 2 layouts.  
• Fixed setting the size of a plot in its format window.  
• When adding a keyword to display in the file list window, it previously did not display the values immediately.
• Custom tokens embedded in other custom tokens previously did not remember changes to their output format.  
• FCS File -> Save previously crashed if the file path did not exist.  
• Set the default resolution for histograms to be 1024.  
• Fixed a problem that caused a crash when copying and pasting 3-d histograms or dot plots.  
• Previously, the Print Setup dialog crashed if there were no layouts loaded.   
• Fixed the format windows to work properly in a multi-monitor display. 
• When exporting to powerpoint, the top plots might be drawn underneath the other plots. 
• Fixed a problem that caused a crash when editing the target field of a compensation item.  
• Fixed a problem that rarely led to a crash when calculating statistics for histograms.  
• Previously, if the number of tick marks on a plot axis was set to 0, there would be a crash.  
• Fixed a problem that caused a crash when editing the target field of a compensation item.  
• Previously, creating a batch print action when no printers were available caused a crash.
• Fixed a problem that caused a crash when modifying the header of an FCS file which was also open on a different page. 
• Previously, creating a batch print action when no printers were available caused a crash.
• Previously, if you used the Set as Default function on a plot, then then next time you reopened the program and created a plot, there could be a crash. 
• Previously, creating a batch print action when no printers were available caused a crash.
• Fixed a problem that caused a crash when you undid the creation of a marker, if the marker was in edit mode. 
• Changing compensation name did not enable OK button.
• If objects on a page were zero height or width, there would be a crash when drawing.
• Stats windows were not updating property if you changed parameters.
• Multiselecting overlays on the Overlays format category caused the program to freeze.

• Fixed a problem related to reading numbers on computer systems where the decimal separator is ",", exhibited while reading in saved layouts.
• Fixed a problem in the concentration calculator setup window.
• The Diva import wizard now imports gate tree views, in addition to plots. 
• Fixed a problem caused by inserting arrows by clicking once in the layout.
• Compensation tokens are now updated when the compensation is modified. 
• Fixed a problem caused by inserting arrows by clicking in the layout.
• Previously, right-clicking on some parts of a token and selecting Format caused an error. 
• Fixed a problem with parameter label sets.
• Fixed a problem that could be triggered by changing the data file of a plot, particularly from a high-resolution FCS 3.0 file to a low-resolution FCS 2.0 file.
• Fixed a bug where exporting FCS files to a file in a folder that doesn't exist caused an error.
• Fixed a problem in the histogram subtraction tool, where gates could not be selected from the drop-down list.  
• Fixed a problem caused by Diva experiment files specifying inconsistent scales for plot axes.  
• Fixed a problem caused by FCS files having negative data values - particularly a problem for Diva data files.  
• Removed the Files in Memory window when selecting FCS File -> Open. 
• Improved error handling of invalid parameter math formulas. 
• Added x and y median to the statistics available in a 2D statistics token. 
• Fixed a problem caused by formatting a plot in a layout that was saved without linking to the data files 
• Several other minor fixes.