FCS EXPRESS: Image Cytometry Application Examples

The Chemometec NucleoCounter makes acquiring DNA content data from slides quick and easy. FCS Express 4 Image Cytometry now makes analysis of your images and getting to results just as simple. Use multicycle to generate powerful cell cycle statistics and batch process your image cytometry data to analyze and archive your results in record time. See for yourself by exploring the layout using the free FCS Express Reader: Chemometec Analysis with Multicycle in FCS Express Image or download a PDF version: Chemometec DNA Analysis PDF. Please visit our knowledge base page to learn more about exporting your data from the NucleoView NC-3000 Software for use in FCS Express 4 Image. Cytometry or our Chemometec Partnership page to learn more about this collaboration.

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The Nexcelom Cellometer Vision allows the user to quickly acquire imaging data for a variety of standard assays including Apoptosis. Your multi parameter imaging data can now be easily analyzed in FCS Express and expanded upon using all of the tools and features FCS Express has to offer. See for yourself by downloading the PDF version of the layout: Nexcelom Apoptosis Assay PDF or by using the free FCS Express Reader: Nexcelom Apoptosis Assay. Please visit our Nexcelom Partnership page to learn more about this exciting collaboration.

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Organizing, analyzing, and presenting large sets of imaging data can be intimidating. FCS Express 4 Image Cytometry allows you to import plate based data segmented in CellProfiler or ImageJ for easy analysis and presentation. See for yourself while learning FCS Express 4 Image Cytometry with our CellProfiler Export and ImageJ Export tutorials You may also explore the layout using the free FCS Express Reader: Nuclear Translocation Assay with CellProfiler Segmentation or download a PDF version: Nuclear Translocation Assay PDF. You may also download the complete ImageJ Data set if you would like to create your own layouts with the data.

FCS Express can be used to organize, analyze, and get results from any imaging experiment. By using CellProfiler to segment your images you can analyze the simplest single image experiments to complex multi-time point and multi-color assays in FCS Express. See for yourself while learning FCS Express 4 Image Cytometry with our CellProfiler Export tutorial. You may also explore the layout using the free FCS Express Reader: Nuclear Translocation Assay with CellProfiler Segmentation or download a PDF version: Research Imaging PDF.

FCS Express can also be used in conjunction with ImageJ to get data from your images. By using ImageJ to segment your images and macros from De Novo Software you can quickly export and analyze your data with FCS Express. See for yourself while learning FCS Express 4 Image Cytometry with our ImageJ Export tutorial. You may also explore the layout using the free FCS Express Reader: Image J Analysis with FCS Express or download a PDF version: ImageJ Research Imaging PDF.

FCS Express can be used to analyze any images and that includes western blots. By using CellProfiler to identify lanes and bands on a western blot image, you can gather band intensity data. You can then compare that data directly to your complimentary image and flow data all in one package. Explore the layout using the free FCS Express Reader: Western Blot and Image Analysis with CellProfiler Segmentation or download a PowerPoint version: Western Blot and Imaging PPT.

You have carefully planned, implemented, and imaged your multi color in-situ staining experiment resulting in large montaged images containing tens or even hundreds of thousands of cells...now what? After segmentation with CellProfiler or ImageJ, FCS Express can handle these large data sets much like a multi color flow cytometry experiment but with the added advantage of spacial resolution and the ability quickly move from the population level down to individual cells and images. See for yourself by exploring the layout using the free FCS Express Reader: In Situ Rat Cerebellum Staining or download a PDF version: In Situ Rat Cerebellum Staining. You may also download the sample data and run the pipeline yourself using the In Situ Rat Cerebellum Staining Sample Data and pipeline.

Images courtesy of Dragan Maric, Ph.D.

Generating large data sets to look at multiple tumor core histology sections has become relatively easy with new imaging technology although bottle necks usually occur during the analysis step. Now with FCS Express 4 Image Cytometry making sense of the data and analyzing it quickly is made easy with the use of interactive plots and batch analysis. See for yourself by exploring the layout using the free FCS Express Reader: Histo Core Analysis or download a PDF version: Histo Core Analysis. You may also download the sample data and run the pipeline yourself using the Histo Core Analysis Sample Data and pipeline.

Images courtesy of Michael Samuel, Ph.D.

The ImageXpress Velos allows you to image and segment entire plates (6 to 1536-well) in less than 5 minutes. FCS Express 4 Image Cytometry now supports Velos data and complements your high speed analysis with in depth multiparametric capabilities. Try for yourself while learning FCS Express with our Exporting from IX Velos tutorial. You may also explore the layout using the free FCS Express Reader: IX Velos Data Analysis in FCS Express 4 Image or download a PDF version: IX Velos Data Analysis PDF.

Colony counting can be a tedious task at best. Let FCS Express 4 Image Cytometry do the counting and classification for you so you can spend more time getting to results. Explore the layout using the free FCS Express Reader: Colony Counting in FCS Express or download a PDF version: Colony Counting PDF. You may also download and run the pipeline yourself using the CellProfiler colony counting pipeline adapted for FCS Express.