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More Testimonials
“Technical support is excellent and the software development team are very keen to react to customer's requirements.”
- Paul Sopp
Division of Immunology and Pathology, Institute for Animal Health, Compton, UK
FCS Express version 4 continues a legacy of innovation in the field by providing
analysis capabilities for 96 well plate format flow cytometry data from
the following instruments:
- BD HTS
- Guava
- Coulter Quanta
- Hypercyte
- Sony
- Accuri
The imported data can be displayed on a per well basis or as an entire plate in Heat Map format.
Heat maps
allow the user to:
- Plot any statistic on any parameter.
- Plot multiple Heat Maps from different files, statistics, or parameters simultaneously.
- Heat Maps can be gated on any gate you like, including by well, and wells can then be individually examined as any other type of plot that FCS Express supports.
- Work with High Content Screening or High Content Analysis data sets
Gates can easily be created on any heatmap through the Gating Tab (below-top row) or by right clicking on a plot and choosing Create Gate>Well (bottom-right). Gates created on a heat map can be applied to dot plots, histogram etc, just like any other gate. The video (bottom-left) also shows heat map gating in more detail.
Heat Map Gating |
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Any parameter or statistic associated with your Heat Map can be displayed on the axis of the plot.
Parameters can be chosen by right clicking on the axis of the Heat Map |
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Just like any other plot in FCS Express, Heat
Maps are fully formattable, including the six different plate color
schemes seen below. Click on the images below to see a larger image.
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| Green-Blue | Rainbow | Red-Black | Grey scale |
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| Inverse Grey scale | Threshold-set a user defined threshold to highlight wells that fall above or below a value. |