In 2005, Union Biometrica wanted to create an Application Note on Analysis and sorting of mouse, rat, pig and human pancreatic islets using large particle flow cytometry using their BioSorter instrument. They wanted to use a flow cytometry data analysis package like WinMDI, FCS Express or WinList. After evaluating the available products, they settled on FCS Express. Rico Bongaarts, Manager of Training and Applications says: "We chose FCS Express because it allowed us to do all statistical analysis we wanted to do and exporting to Word and PowerPoint is very easy. FCS Express is very intuitive and we have chosen to purchase a copy and distribute it with every instrument we sell in order to provide our customers with the highest quality data analysis tools.”

"As Medical Director of the Flow Cytometry Laboratory of the Shands Teaching Hospital at the University of Florida, I have been involved with flow cytometric data analysis and teaching for more than 20 years. Throughout this time I have used many different software packages to analyze and display flow cytometry data. I have been using FCS Express for several years and am extremely pleased with its functionality and flexibility. No other software package is as powerful while remaining user-friendly that can be learned rapidly and intuitively. It provides a great number of options for analysis and offers multiple display possibilities. The electronic Manual and Tutorial are superb and, above all, the support available is exceptional."
- Raul Braylan
Medical Director, Flow Cytometry Laboratory of the Shands Teaching Hospital at the University of Florida

"I have finally finished the analysis of my experiment in FCS express. I took the time to read the manual and try to test all the features available. I found the program incredibly useful! I did not know if to laugh or to cry when after finishing the "untreated" set, i just hit "ctrl-alt-arrow" as needed and in 2 min had a perfect analysis for my LPS treatment. I can't believe we were so foolish to wait so long before ditching the software that came with the flow cytometer."
- Uriel Trahtemberg
MD/PhD student, The Hebrew University - Hadassah Medical Organization

"I manage a flow laboratory equipped with three BD cytometers and have been using FCS Express for several years, since the release of the first version. FCS Express provides an intuitive, layout-based organizational format, allowing users to visualize plots and statistics on the same layout. I’ve used most of the flow software programs out there and find FCS Express to be at the top of my list for several reasons, one of which being it can be used to analyze many different types of flow data. FCS Express has most of the features found in the more expensive commercial software programs and costs a fraction of the price. There are also some features in FCS Express that are not found in other programs as well as some features that function better, such as the Undo feature – which I find to be very useful when editing plots. The programmers keep the software up to date and quickly fix any problems/bugs, and the customer service and technical support staff are very knowledgeable and helpful (although I have had to contact them only a couple times over the years). Overall, I highly recommend FCS Express to both new and veteran flow users."
- C. Mac Trubey
Manager, FACS Laboratory

"When I started using flow cytometry 4 years ago, I searched for a usable, affordable, PC-based analysis program. I looked at many programs and FCS Express (Version 1.0) came out on top. Over the past 4 years, I have continued to evaluate FACS software packages, FCS Express continues to be full-featured, flexible, and user-friendly, at an affordable price. If that isn't enough, De Novo Software's tech support is incredible; in addition to fast and friendly technical support, the team at FCS Express listens to its users' suggestions and works hard to integrate those ideas to improve the product. I can't say enough good things about the program and the people who work on it, and I recommend the program to everyone who asks how I work up my data."
- Lisa Kohli
Laboratory Technician, Indiana University

"Having approximately 60 users, who vary from novice to expert we needed an analysis package that can cater for a wide range of competency. FCS Express does this , it is well suited for a first time user as well as complicated analysis by the more experienced. The ability of the “networked version” to run the program from the server allows us to make the program available throughout the entire institute , ensuring everyone has access to a flow analysis package. To “end users” the ability to export directly into PowerPoint is a substantial benefit as are the other many other features ,eg, file list, multiple pages, legends in overlayed histograms, etc. The different versions of the program that are available is also a good feature, ie, the inexpensive “Lite version” which is good for home use, through to the standalone and the networked versions. The support provided by “De Novo Software” has been excellent, they have shown a willingness to listen to the users' needs, in fact they have been “pro active“ in this respect, also they respond to these needs in an appropriate time. There is also an “on line” forum . This support denotes a company that is continually improving what they provide and is willing to communicate directly with users."
- Ralph Rossi
Manager Flow Facility

"Since its release, FCS Express Version 2 has become an indispensable tool for analysis of flow cytometry data in my laboratory. Version 3 has only enhanced an already strong program. Its interactive batch processing capabilities are both intuitive and extremely flexible. I particularly appreciate the ability to track any keyword or statistical parameter and to place these into Excel spreadsheets with custom labels. Entire layouts can be fully annotated and easily exported to Powerpoint files during batch processing. I have found this to be an enormous benefit when rechecking the quality and consistency of my data over time. Version 3 has an even more professional look and feel.  There are more options for two-color displays, and even more versatility for data analysis.  The attributes of multiple plots can now be changed with a few clicks of keystrokes.  The price remains lower than other flow cytometry analytical programs, and support has always been outstanding. This will continue to be my flow cytometry program of choice."
- Eric Sobel
Associate Professor of Medicine, Division of Rheumatology & Clinical Immunology, University of Florida

"The most important quality of the program is its evolution. The program is constantly being expanded, modified and refined to make it more convenient and easy to use. What made this program stand out over others is the immediate responses from the staff, and the frequent updates that take into consideration the users' feedback. I can't think of any other software company that does this."
- Deniz Top
PhD student,Department of Microbiology and Immunology, Dalhousie University

“I have been processing flow cytometry data since the days of Polaroid capture of the oscilloscope traces. Having tested just about every major platform available, I am confident that De Novo Software has the product that truly outperforms the rest. FCS Express V3 combines exceptional flexibility in data analysis with unparalleled ease of use. We routinely perform batch processing with exports to Microsoft Excel and Powerpoint. Appending header information to a statistics result has made data processing fast and simple.  Exported results can now be queried, filtered, sorted and summarized to generate experimental tables that make sense. The graphics are publication quality and are infinitely configurable to meet electronic submission requirements of most major journals. Installing the software on our network allows multiple users to share templates and eliminates the maintenance of multiple stand alone versions with every release. Every flow laboratory has a software wizard that can process flow data but now every member of my laboratory is a flow data processing wizard! ”
- David DiGiusto Ph.D. 
Director of Analytical Cytometry, Beckman Research Institute of the City of Hope National Medical Center

“At the Institute for Animal Health, Compton, between 40 & 50 flow operators use FCS Express V2 Professional Network installation. This has proven to be an extremely economical solution to our data analysis requirements. The Institute generates large numbers of samples so efficient batch processing is critical – the ability to print/save layouts, save statistics to Excel and create presentation quality output straight into PowerPoint has boosted productivity enormously. Technical support is excellent and the software development team are very keen to react to customer’s requirements.”
- Paul Sopp
Division of Immunology and Pathology, Institute for Animal Health, Compton, UK

"FCS Express allows for the rapid analysis of flow data in a very customizable environment.  With programmable statistics reporting and very flexible plot duplication features, that include reading data from sequential fcs files, large experiments can be gated, plotted and analyzed in a printable format with very few steps.  You won't have to set aside big chunks of your day to analyze flow data."
- Marc Gavin
Assistant Professor, Dept of Immunology, University of Washington, Seattle. 

"The web site gave me the option of downloading at the end of the order. I did that and was fully impressed that the program loaded, went to the web and installed itself promptly. ... You ought to license this installation process out! It seems pretty darn dummy proof."
- Louis King, Ph.D.
Manager Flow Cytometry Core, Research Technology Support Facility, Michigan State University