If you have any questions which do not appear on this list, please do not hesitate to contact us directly.

  1. What operating systems does FCS Express run on?
  2. What is your refund policy?
  3. What is a configuration file?
  4. What is a countercode?
  5. What is the difference between the demo, lite and professional versions?
  6. I have saved my layout as a PowerPoint presentation. Can I edit the text in the text boxes or on the plots?
  7. Why are there access violations when I save and load files?
  8. Why does the text on the right-most x-axis label cut off on my plot?
  9. How does FCS Express implement software compensation?
  10. How can I track a population in FCS Express?
  11. Why do I get errors upon exiting FCS Express?
  12. My computer which was running FCS Express had to be replaced. How do I reinstall FCS Express on my new machine?
  13. Why does FCS Express crash immediately upon starting up?
  14. Where is the configuration information for FCS Express stored?
  15. How can I set up FCS Express to read data saved by an Abbott CellDyne Sapphire?
  16. How can I set up FCS Express to read data saved by an Accuri C6?
  17. What are the Resolution Options on the General Category of the plot formatting screen?
  18. Why do my dot plots appear sparse and/or blocky?
  19. Why does the compensation on my Coulter FC 500 data seem incorrect?
  20. Why won't FCS Express recognize the dongle after I've inserted it in the USB port?
  21. Why does my Windows 7 computer crash after installing the dongle drivers?
  22. Why do I get a connection error when I try to use DDS or Publishing?
  23. How much memory (RAM) can FCS Express use?
  24. How can I best display my FCS 3.0 data acquired in BD FACSDiva?
  25. What is Biexponential and Hyperlog?
  26. What is the best way to display my FCS 3.0 data acquired in BD FACSDiva on a 4 decade log scale?
  27. Why do I get the message that a data file exported from a Diva experiment is invalid?
  28. Why is my Geometric Mean Always Zero?

How to display BD FACSDiva data in 4-decade log

What is the best way to set FCS Express to display FCS 3.0 data from FACSDiva on a 4 decade log scale?

The FACSDiva™ software gives you a choice of displaying your data in 4- or 5-decade log scale. When you choose to display FACSDiva data in log in FCS Express, it defaults to display 5 decade log. However, you can easily change your settings to display 4 decade log.

Right click on the plot and select Format. Set the resolution options (found in the General category) as shown in the image below. Make sure to set both the X- and Y- axis.



An example of the same data shown on biexponential and 4 decade log
Biexponential scaling4 decade log scaling

Notice in the data above that there is quite a lot of data below channel 26 (the lowest channel  included on the 4 decade log plot). The include out of range values in gates and quads allows you to choose whether events below the axis are included in gates and quads drawn on the log plot.

AB
C

Gate 1 was drawn on a 4 decade log plot with the include out of range values in gates and quads checked (A, above). The plot was then changed to show biexponential scaling (B, above), which shows all the data. Note that there are red cells to the left and below the border of the gate. They are colored red because they are considered to be part of Gate 1, since the plot upon which gate 1 was drawn had include out of range values in gates and quads checked.  If Gate 1 was drawn on a plot with include out of range values in gates and quads unchecked, the events below the axis would not be included as part of Gate 1, as shown in C.

Quadrants statistcs are similarly affected. In the left panel below, the quadrant statistics add up to 100%, since the events below and to the left of the plot are being included as part of the quadrant calculations. In the panel on the right, the quadrant statistics do not add up to 100% because cells with values below 26 (the lowest value being displayed on the plot) are not included in the quadrant statistics.
Quads drawn on 4 decade plot with include out of range values in gates and quads checked.Quads drawn on 4 decade plot with include out of range values in gates and quads unchecked.

Please note if you set the resolution options as described in the first figure above, the Axis Ranges can all be set to automatic, as in the figure below:

 FACSDiva is a trademark of Becton, Dickinson and Company

 
Copyright © De Novo Software 2009. All rights reserved. Privacy Policy Terms of Use
De Novo Software