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This page allows you to download the latest release of FCS Express 6 - Research version. To upgrade to the latest IVD version please click here.

To upgrade to the last release of FCS Express 5, please click here.

To upgrade to the last release of FCS Express 4, please click here.

The current FCS Express release is 6.01.0010.

FCS Express 6 for Windows - 64 Bit

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FCS Express 6 for Windows - 32 Bit

Download FCS Express 6 Flow Cytometry for Windows - 32 Bit

Download FCS Express 6 Image Cytometry for Windows - 32 Bit

Download FCS Express 6 Plus for Windows - 32 Bit

FCS Express 6 for Mac

Download FCS Express 6 Flow Cytometry for Mac

Download FCS Express 6 Image Cytometry for Mac

Download FCS Express 6 Plus for Mac

Release Notes

Version 6.01.0010 - December 8, 2016 (Research Use Only)

Bug Fixes

  1. Previously, when the Windows CHM help file was open beside a layout, searching in the CHM would sometimes lead to a crash
  2. Fixed a crash that occurred while sorting the Data List
  3. Fixed a crash that could occur on the Mac when selecting a file from the "Recent Places" list
  4. Fixed a crash that occurred when loading a layout saved with Data-Specific gates, if the original data files were missing and the data files were replaced during loading
  5. Fixed a problem that was introduced in 6.01.0009 that caused log-based color levels in density and contour plots to be displayed as linearly scaled if there were no negative numbers in the values
  6. Prevented a crash that occurred when editing compensation manually, if a blank was entered into the compensation value field
  7. Prevented a crash that could occur when using bar plots to plot spreadsheet data, if there was an invalid formula in the spreadsheet
  8. In version 6.01.0009, if you were updating from the previous version, the DNSEABridge executable was not replaced with the newer version - this has been fixed
Version 6.01.0009 - November 21, 2016 (Research Use Only)

Bug Fixes

  1. Previously, when setting up quadrant authorizations, checking was not performed to prevent circular references
  2. Shortcuts on Mac now correctly use the Command key
  3. Fixed functionality for the printer icon on the quick access toolbar, and made sure that the print dialog was initialized correctly
  4. Prevented crash when performing parameter math using templates specifying non-existent files
  5. Fixed a crash triggered by data-specific gates when loaded in a layout where the data files could not be found
  6. Fixed a crash that occurred on heat maps when a gate had points with negative values
  7. Fixed copying and pasting of cells between spreadsheets on the layout
  8. Prevented a crash when attempted to calculate PCA using a gated subset that has no events
  9. Previously, permission rule formulas that included certain tokens did not always display correctly when the layout was reloaded
  10. Fixed a crash that occurred when adding a new compensation while the previous compensation matrix was being edited
  11. Changed the default folder for the auto-recovery file on MacOS to be in a standard folder
  12. Removed the incorrect % sign from density plot legends
  13. Fixed reordering items in the SOP list using drag and drop - previously the drop would put the item in an incorrect position in the list
  14. Fixed a crash that could occur in the Gate Navigator when the option to show plot nodes was turned off
  15. Improved the stability and error handling of the external application bridge
  16. Kinetics stats items now handle their gates being deleted gracefully without crashing
  17. Improved the way FCS Express interacts with MacOS in handling messages from the operating system
  18. Improved stability on MacOS
  19. Previously, spreadsheets changed their selected cell after zooming
  20. Disabled exporting data from spreadsheets using the ribbon Export button
  21. Eliminated an unnecessary Paste Special dialog when dragging cells from a data grid onto the page
  22. More UI fixes for high-resolution displays
  23. Spreadsheets no longer appear in the data list
  24. Prevented adding inappropriate statistic tokens for pie charts
  25. Improved Undo/Redo for changes inside a spreadsheet
  26. Simplified the behavior when dragging batch actions to reorder them
  27. Previously, FCS Express was unable to reopen layouts files referring to ICE data if the data had been moved on disk
  28. Removed transformations from the list of data that can be selected in the Export Data dialog - the transformations can be chosen separately in that dialog
  29. Made sure that compensation matrix tables in text boxes are displayed correctly even when the layout is set to remember parameters by name rather than number
  30. Simplified dragging vertices from one marker to another for creating links
  31. In the previous release, on MacOS a folder could not be selected after setting the data file type to FCS Plate Folder
  32. Fixed a crash that could occur when editing spreadsheet cells after merging cells
  33. Prevented changing plot types on plots that have no data loaded in order to avoid a crash
  34. Prevented sizing from being doubly applied when resizing pictures using the format window after using the Preview button
  35. Fixed a crash that could occur when dropping down the list of items in a token drop-down list
  36. Prevented spreadsheets from changing to a data file when using the Change on All buttons on the ribbon
  37. Ensured the pie charts update correctly if they are displaying spreadsheet data and the spreadsheet is on a page that's not currently visible
  38. Fixed a crash that could occur after renaming an SOP step without pressing Enter
  39. Previously, copying and pasting text with bullet items from PowerPoint into FCS Express caused a crash
  40. Fixed a crash that could occur in tSNE transformations if a parameter had no values
  41. Previously, using the configuration wizard with a network version to select a configuration file that was already saved on disk led to an error message
  42. Made tSNE calculations more robust
  43. Previously, on MacOS, selecting parameters on a plot in a list box that required horizontal scrolling was difficult to perform
  44. Fixed a crash that occurred in layouts showing Nexcelom data after opening an empty template and loading data into it
  45. Improved performance when opening the System tab in the Ribbon Backstage
  46. Fixed the usage of the default folders specified in the Options (previous it was sometimes using the most recent folder instead of the default)
  47. Previously, when plotting data from spreadsheets on dot plots, gates on those plots would include 100% of the data regardless of their position
  48. On MacOS, maximizing FCS Express now leaves room for the Dock
  49. After opening modal dialogs from the Ribbon Backstage, the title bar on the main window of the layout is now redrawn again properly


  1. The BD Accuri importer can now import from .CI files as well as .C6
  2. Dragging a marker from one plot to another now creates a linked marker on the second plot
  3. Added a button for explicitly executing R scripts
  4. Parameter Math sequences from version 4 and 5 layouts are now imported into a new Parameter Math transformation.
  5. Improved storing transformation template files so they can be saved with relative paths
  6. Reduced the sensitivity of the mouse scroll wheel for MacOS
  7. Formatting quadrant text boxes now starts on the Contents category
  8. Improved handling of defaults for batch items in reports with field labels, where the field label has not been explicitly set
  9. Signing electronic signatures now requires entering the user name as well as password the first time in a session (subsequent signings only require the password)
  10. Errors the occur while performing R scripts and other transformations are now added to the layout's log (accessible on the status bar of the layout)
  11. Added an option for displaying histogram overlays in the legend using a box instead of a line
  12. The option for dithering colors on color dot plots has been changed to default to false
  13. Added coloring in compensation matrix tables in text boxes
  14. Dragging a marker onto an empty part of the page now inserts a text box and token, like it already did for gates
  15. Dragging data files onto a spreadsheet cell now pops up a dialog for selecting a stat or keyword token
  16. Added the ability to click on gate or quad vertices in a plot and select them even if they are "underneath" another gate
  17. Improved the usability of the crash dialog
  18. Improved the spacing between the labels and titles on vertical axes on plots
Version 6.00.0053 - September 24th, 2016 (Research Use Only)

Initial release of FCS Express 6