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Enhancements

1. Internet dongle enhancements.

Enhancements

1. Added support for the ImageJ data format.
2. Added support for ICE format version 1.1.
3. Improved stability and speed of internet dongle and site license servers.
4. Improved the speed of dragging and dropping plots.
5. Added new functions RemoveText and ReplaceText in custom tokens.

Bug Fixes

1. Fixed a problem that prevented people with network licenses from adding new users.
2. Fixed a problem with clinical launch files using layout map IDs.
3. In cases where custom tokens contained other custom tokens, deleting them would sometimes cause the program to hang.
4. Renaming custom tokens in some cases would cause a crash.
5. Improved the handling of Z-order when copying a group of plots to the clipboard and pasting.
6. Reenabled right-click->Delete on batch actions.
7. Fixed a problem with saving the "below zero" value of a biexponential formula - previously, if the value was saved as 0 and the layout was saved, it was sometimes reset to 200.
8. Resolved a crash that happened when deleting a linked gate.
9. Reenabled deleting non-admin users in the Internet Dongle license.
10. Improved the speed of setting up the Email to Notify in the security configuration window and fixed a crash that occured on invalid entries.
11. Fixed a crash that happened in very large layouts when deleting a gate that was visible or used on many other plots.
12. Improved the messages shown when building histograms for automatic compensation.
13. Fixed undoing assigning compensations to a plot through dragging a compensation.
14. Eliminated problems that happened when undoing signing a layout.
15. Fixed a Windows resource problem that occured when using the navigator windows in very large layouts.
16. Sped up typing in the Alerts windows.
17. Fixed a crash that happened when double-clicking on a layout icon on the desktop, if that same layout was already open in FCS Express and was in the process of being closed.
18. Allowed entering values lower than 90 for the JPG quality setting.
19. Fixed a problem that caused files in the data list to be loaded in a non-alphabetical order.
20. Prevented a crash when moving linked gates in a Gate View.
21. Forced tokens to update to empty values when the data list is cleared, and the plots that the tokens are pointing to become empty.
22. Prevented parameter math from running on embedded data files, when the option is set to run when files are loaded.
23. Fixed the file open dialogs to properly display Windows folder shortcuts.
24. Previously, outliers were not always drawn correctly for each outlier in contour plots where the palette is set to use logarithmic levels.
25. Fixed a problem that caused Multicycle plots to perform an incorrect fit if the data was magnified.
26. Resolved a memory leak that occured when copying plots to the clipboard.

Enhancements

1. Sped up plot display when adjusting manual compensation.
2. Added copy protection information (countercode, dongle, internet dongle) to the About window.

Bug Fixes

1. Fixed the problem that caused layouts saved or published from inside a batch processing report to lose plots on the 2nd pages.
2. Fixed the duplication of keywords that occured on certain layouts after publishing a layout inside a batch process report.
3. Fixed setting permissions on a published layout inside a batch processing report.
4. Resolved a crash that could occur in the standalone license when the computer was waking up from being asleep.
5. Restored the ability for network users to double-click on layouts and open them immediately.
6. Fixed the display in the About window that caused standalone licenses to appear to be network.
7. Resolved display problems in the automatic compensation setup messages.
8. Fixed a crash that could occur when dragging and dropping a compensation matrix in a text box, if there was already other text in the text box.

Known Limitations

1. Network users will lose the ability to double-click on layout files, and automatically open them in FCS Express. Instead, you will need to first open the program, then open the layout. This will be resolved shortly in an upcoming release.
2. If you are publishing the layout inside an XML in batch processing, then if there are hidden pages at the beginning of the layout, the plots on subsequent pages may not appear in the layout. This will be resolved shortly in an upcoming release.
3. If you are using the security module with an ElevateDB database, the upgrade will not be backward-compatible. Please contact us for assistance.
4. Internet dongle users are required to upgrade.

Enhancements

1. Revamped and improved compensation infrastructure. There is a new dockable window with compensations, and an improved method of setting up automatic compensation.
2. Improved management for the security system and internet dongle users, including the ability to log people out remotely, and to set up notifications for login failures or other events.
3. Improved memory usage for picture plots.
4. Improvements to the Proliferation fitting algorithm.
5. Improvements to the contour plot outliers.
6. Added options for gate text box placement.
7. UI Enhancements: new buttons for zooming, and fewer clicks required to log out and change preferences.
8. Implemented Ctrl + mouse wheel for zooming.
9. Additions to the manual for Electronic Signatures
10. Added options for changing the Edit/Selection colors of plots
11. Improved speed of editing Users and Groups in the security module.
12. Introduced a caching mechanism, to improve the speed of starting up FCS Express when running from a network drive.

Bug Fixes

1. Fixed a problem that led to statistics not updating immediately if a plot had percentile quadrants, and the gate that the plot was gated on was modified, or the data or compnesation was modified, and the plot was not showing on screen.
2. Resolved a crash that could happen if bad information was on the clipboard.
3. Previously, dragging and dropping text boxes into plots did not work.
4. Resolved crashes when importing certain CFlow files.
5. Eliminated a crash that sometimes happened if the monitor turned off while FCS Express was running.
6. Fixed a problem that led to a picture being specified as having 0 width in an XML export
7. Fixed a potential hang after exporting to Excel.
8. Fixed an occasional crash that happened when moving gates in plots with biexponential axes.
9. Other stability improvements

Enhancements

1. Added the ability to read Nexcelom data files.

Fixes

1. Fixed a problem introduced in 4.00.0053 where the database repositories could not be installed.
2. Fixed a problem where plots based on custom data using tokenized strings were not always updated when the layout was loaded.

Enhancements

1. Added the ability to read data in the ICE and ACS standard.
2. Improved memory management of image data - loading image data sets is no longer limited to the amount of available RAM.
3. Added SSMD and Z-Prime
4. Now supports having more than one config file in the program settings folder - you will be prompted for which configuration to use.
5. PDF version 1.6 is now supported in PDF batch reports.

Fixes

1. Fixed a bug where quad stats did not immediately update visually when Pause between iterations was turned on in Batch Processing.
2. Improved handling of scatter parameters from iCyt and InFlux data files so that they are displayed in linear by default.
3. Renamed references to Isocyte from Molecular Devices to be ImageExpress Velos.
4. Improved how the gate color precendence is calculated in dot plots.
5. Previously, when adding an overlay to a histogram, the compensation was not automatically applied.
6. Fixed reading in the parameter name keywords from the second data set of Beckman Coulter data files.
7. Fixed a crash in drawing gates if the gate was too small.
8. Fixed problems with Multicycle and CellProfiler data when used with Windows regional settings where the decimal separator is not '.'
9. Enabled resizing columns in data grids.
10. Enabled changing plot types for plots imported in version 3 layouts.
11. Fixed a problem in exporting data files more than once.
12. Uninstall now removes the program settings folder where possible.
13. Improved the way range selection is calculated in Cell Number gates on data grids.
14. Improved the appearance of options dialogs and wizards if Windows is set to use large fonts.
15. Resolved a crash that happened when you started up a network version but did not have proper permissions on the network share.
16. Previously, the % Positive statistic was not always recalculated on time.
17. Improved the way the CFlow page options are handled during import.
18. Ensured that histogram overlays from a CFlow import are all colored uniquely.
19. Improved handling of dragging and dropping tokens onto plots.
20. Fixed a crash that could happen if Office was not installed on Windows.
21. Reduced flickering when navigating through tables inside text boxes.
22. Fixed a bug that the parameter label settings were not always remembered in the axis label formatting category.
23. The active state of batch processing actions is now remembered properly.
24. Fixed the ability to magnify heat maps.
25. Removed the program expiration date of Jan 1, 2012.
26. Improved handling of Office .docx, .xlsx and .pptx files.
27. Stopped requiring validation of batch actions that were deselected.
28. Fixed a crash that occured if percentile quads were set to 100%.
29. Fixed the legend color in bar plots.
30. Improved the way axis labels are shown in surface plots.
31. Other stability fixes.

Enhancements

1. Added several regression models: 5PL, 4PL, two-phase exponential fit, power series, Gaussian, Bolzmann, exponential association, sine wave, two-site binding, parabolic, cubic
2. Added a node for plots with "No Data" in the Data Navigator.
3. Enabled resizing columns in data grids.

Fixes

1. Improved support for Multicycle in languages with a comma as the decimal separator.
2. Changing parameter names in the header did not propagate changes to open plots.
3. When dragging a data file into a histogram to create an overlay, an undo was not added to the undo system.
4. Fixed a crash when you try to open the Axis formatting options on the ribbon for a bar chart.
5. Improved stability when right-clicking in table cells and placeholders.
6. Improved stability with Isocyte data when switching options between loading and not loading images.
7. Fixed a crash that happened when closing a layout that had several dockable windows docked together.
8. Previously, if a plot was using a parameter in a data file that no longer existed, it could trigger a crash.
9. Fixed autosizing of statistics windows when loading version 3 layouts.
10. Other fixes for loading layouts from version 3.
11. Fixed a crash that could happen when displaying a log parameter with a non-automatic range.
12. Fixed a crash when deleting an overlay that was used to normalize another one.
13. Previously, dragging a file from the data list off and then back on to the list sometimes caused a crash.
14. Fixed a problem when dragging a plot from one layout to another.
15. Previously, FCS data files larger than 100 MB could not be written properly.
16. Previously, if an Excel report specified a worksheet name which was blank or only had tokens in it, this could lead to an error.
17. Fixed a problem with the Accuri import when trying to import histograms which had overlays, but not all overlays had data files.
18. Fixed the page layout calculations in the Accuri import wizard.
19. Other stability fixes.

Enhancements

1. Added the ability to take layouts saved in FCS Express 4 Plus and open them in FCS Express 4 Flow.
2. Allowed Word batch processing export to use more than 60 merge fields.
3. Added a warning dialog box when you save version 3 layouts.
4. Updated the program icons to be distinct between Flow, Image and Plus.

Fixes

1. Tokens for Multicycle statistics on a different page from the original plot did not always update in time.
2. Multiselecting histogram overlays in the Overlays formatting category did not allow you to change the parameter.
3. Previously, files without extensions were not being shown in the open data dialogs.
4. Improved the way overlay lists are combined in the Quadrant and Overlays formatting categories when multiple plots are selected.
5. In the Diva import, if a tube name had characters that were invalid for a filename (ie ;?/\) it was not displayed properly in the dialog that asks to select the acquisition filename.
6. In the Diva import, if the files were located in a different folder than the XML file, then they were not found even if you selected them in a dialog.
7. In the Accuri import, the axis options are now set to automatic by default.
8. Fixed the background color property for picture plots.
9. Fixed a crash that happened if you were in the middle of renaming a custom token and closed the layout.
10. Fixed a crash in deleting multiple cells in a custom data grid.
11. Fixed loading the background image for picture plots.
12. Resolved a crash when displaying bar plots with a log axis.
13. Fixed a problem with setting permissions for publishing using the advanced publisher.
14. Fixed a crash that happened when the Select User dialog is open, and you double-click on a layout file in Windows Explorer.
15. Prevented a crash in the Align feature when plots are grouped together.
16. Fixed a problem changing the legend text in the Edit Preferences window.
17. Prevented a crash when deleting position markers in Multicycle plots.
18. Prevented a crash when you drag 2 or more files from Windows explorer into the Data List.
19. Previously, changing the order of the batch actions was not always remembered.
20. Improved the program behavior when you switch the number of monitors on your computer.
21. Prevented a crash when you copy and paste a gate onto a plot from a plot showing different parameters.
22. Stability fixes when loading in version 3 layouts.
23. Other stability fixes.