Download Latest IVD Version

FCS Express 5 IVD is the most recent IVD release.

The final FCS Express 4 Flow Cytometry IVD release is 4.08.0016

Download FCS Express 4 Flow Cytometry IVD

Release Notes

Version 4.08.0016 - October 28, 2016 (Clinical-Only)

Bug Fixes

  1. Removed kinetics and proliferation plots and statistics from FCS Express 4 IVD.
Version 4.08.0015 - March 30, 2016 (Clinical-Only)

Bug Fixes

  1. Ensured that on plots with calibration turned on, quad values are shown with calibration.
  2. Fixed a problem that caused percentile quadrants on layouts loaded via launch files to not be initialized correctly
Version 4.08.0013 - August 3, 2015 (Clinical-Only)

Enhancements

  1. Added features for specific clinical customers.
Version 4.08.0012 - March 18, 2015 (Clinical-Only)

Bug Fixes

  1. Fixed a problem that cause the Data List to be blank on a newly opened layout, if the layout was opened using a launch file, and the Data List was set up to be open initially.
  2. Fixed the SMTP configuration options to allow Authentication Type of None.

Enhancements

  1. Added features for specific clinical customers.
Version 4.08.0010 - January 28, 2015 (Clinical-Only)

Bug Fixes

  1. Reverted a change in color levels in density plots introduced in 4.08.009 so that color levels again look the same as 4.08.0008 - the fix for outliers introduced in 4.08.0009 was retained.
Version 4.08.0009 - January 9, 2015 (Clinical-Only)

Bug Fixes

  1. Fixed a display problem in density plot with outliers where cells with a value > 1 but less than the cutoff value for calculating levels were not drawn.
  2. Removed the prompt for the preferences password when automatic changes are made to the preferences during batch processing.
  3. Fixed a problem startup up the network version of FCS Express on very slow networks.
  4. Minor changes for specific clinical customers.

Enhancements

  1. Added a function to custom tokens to enhance reading the date from the FCS Standard $Date keyword, called DateKWToDate.
Version 4.08.0008 - September 5, 2014 (Clinical-Only)

Bug Fixes

  1. Fixed a problem displaying parameter keywords for Beckman Coulter data files when the parameters don't match up exactly between the FSC 2.0 and 3.0 datasets inside the LMD.
Version 4.08.0007 - June 20, 2014 (Clinical-Only)

Bug Fixes

  1. Fixes for specific clinical customers.
Version 4.08.0006 - June 12, 2014 (Clinical-Only)

Enhancements

  1. Added the ability to select macro-enabled Excel files in the file dialogs in Excel reports.

Bug Fixes

  1. Fixed a problem where outlier dots on density plots disappeared after switching to another page and back.
Version 4.08.0004 - April 30, 2014 (Clinical-Only)

Enhancements

  1. Additional functionality for specific clinical customers

Bug Fixes

  1. Stats from kinetics plots did not remember their gate information
  2. In the previous releases, if a token font was based on a token, and that token updated, the token font did not always update
  3. Resolved problems reading keywords from Beckman Coulter Gallios and Navios FCS files.
Version 4.08.0003 - November 14, 2013 (Clinical-Only)

Bug Fixes

  1. Fixed a crash when dragging a gate to the Autocompensation setup window from a Gate View.
  2. Fixed a crash that happened in some cases when changing to a Lin/Log scale.
  3. Fixes for some custom reports for clinical customers
  4. Resolved problems reading keywords from Beckman Coulter Gallios and Navios FCS 3.0 files.
Version 4.08.0001 - September 25, 2013 (Clinical-Only)

Important Upgrade Announcement

  • This release introduces modifications to the structure of the FCS Express Security database. If your license is configured to use a Security system, please read these instructions carefully before upgrading.

Bug Fixes

  1. Fixed a database problem that led to some people not being able to log in temporarily. This bug only affected customers using the SQL Server database for security.
  2. Eliminated a crash that occasionally happened when re-calculating the automatic compensation, after dragging a gate from a GateView to change the compensation setup.
  3. Eliminated a crash that happened when changing certain histogram axes to the Lin/Log scale formula.
Version 4.07.0011 - August 22, 2013 (Research and Clinical)

Enhancements

  1. Added support for reading plates from Guava instruments into heat maps.

Bug Fixes

  1. Eliminated a crash that happened when undoing a gate replacement, if the replacement was drawn on different axes than the original gate.
  2. Eliminated a crash that happened when opening a layout that had no pages.
  3. Updated export formats for specific clinical customers
Version 4.07.0005 - June 17, 2013 (Research and Clinical - all Enhancements and Fixes from the previous Research-only releases are included)

Enhancements

  1. Optimized loading image data to improve speed.

Bug Fixes

  1. Fixed a bug in the 5 Parameter Logistic (5PL) equation
  2. Fixed a problem with displaying ICE-format data, where cells outside a gate were not drawn correctly on the picture plots
  3. Updated export formats for specific clinical customers
Version 4.07.0003 - May 24, 2013 (Research-only release)

Bug Fixes

  1. Eliminated a crash that sometimes happened while moving polygon gates.
  2. Eliminated a crash from accessing the data source of tokens that were updated from data files, in the case where the layout had been saved unlinked.
  3. Eliminated a crash that could occur if FCS Express was in the middle of scrolling a page, and the computer or monitor went to sleep.
  4. Eliminated a crash that appeared in some cases while the compensation window was open and displaying a matrix, if the user clicked quickly on a different grouping of compensations in the list above.
Version 4.07.0001 - April 29, 2013 (Research-only release)

Bug Fixes

  1. Updated handling filenames of wells from data files inside BD Accuri C6 files to work with the latest versions of C6 software.
  2. Resolved a crash that happened if you created a linked gate from a gate with children, and unchecked the checkbox to Create linked child gates.
  3. Resolved a crash in batch processing which happened when you first saved a PDF file, then reran batch and appended to the same file.
  4. Fixed a bug in the 5-Parameter Logistics regression model.
  5. In previous versions, when doing a batch processing report where the target is an existing file, if the batch report was organized inside a folder in the batch window, the setting of the checkbox for "Ask for confirmation" was ignored.
  6. Improved error handling for the Cell Profiler file loader when it encounters unknown file types.
  7. Added more error checking on temporary files created when generated batch processing reports.
  8. Previously, when parameter math was set to run on loading new files, then if plots were created for new files by where dragging files from the Data list (or from Windows Explorer), parameter math was not run.
  9. Improved diagnostic information on moving gates.
  10. Fixed a crash that could occur when changing axes to use Log with Negative scaling, if there were many events along the axis.
Version 4.06.0006 - March 8, 2013

Enhancements

  1. Molecular Devices MDCStore data is now available for all Image and Plus customers.
  2. Improved the appearance of density plots imported into FCS Express from C6 files.

Bug Fixes

  1. Resolved a problem introduced in 4.05.0005, which prevented certain pictures from being displayed correctly on layouts.
  2. Fixed a crash that occasionally happend when moving or resizing a gate, if the the X parameter on the plot was displayed in the Biex transformation, and the Y parameter was displayed in linear.
  3. Fixed a bug that prevented layouts saved with embedded Acapella data from being reloaded.
  4. Fixed a crash that occurred after deleting a compensation from the compensation window, then dragging and applying a different compensation to a plot that had previously been showing the deleted compensation.
  5. Fixed a crash in the Data List, which happened in some circumstances after loading a layout unlinked, if the data list was set to sort based on keywords.
  6. Fixed a crash that occurred if there were errors when running parameter math, if the layout was set to run parameter math on newly loaded data.
  7. In 4.05.0005, the Collaboration portal did not always start correctly in network licenses of FCS Express.
  8. Resolved a bug in exporting data, if you selected a number of cells to export greater than the number of cells in the data.
  9. Previously, after pasting a marker into a histogram, if the stats grid was open, then it did not get immediately updated with stats for the new marker.
  10. Previously, if a layout was set to run parameter math on newly loaded data, and it also had a panel applied, then if there were errors during parameter math the data would not be displayed in plots.
  11. Other stability improvements.
Version 4.05.0005 - January 15, 2013

Enhancements

  1. Added support for Perkin Elmer Acapella data.
  2. Added support for Molecular Devices MDCStore data.
  3. Added Export to LabKey Server Batch Processing report.
  4. Updated the dongle drivers for Windows 8.
  5. Added functionality for specific clinical customers.

Bug Fixes

  1. Fixed problem in parameter math that would cause it to stop if a division by 0 was encountered in one of the operations.
  2. Fixed a crash when replacing cell number gates.
  3. Previously, undo of performing parameter math was not causing the plots to be refreshed.
  4. Improved error messages in image parsers.
  5. Made the concentration calculator more robust for handling ETIM and BTIM keywords from the Canto Clinical software.
  6. Previously, settings for dialogs with Do Not Show Again checkboxes were not remembered.
  7. Fixed a problem with copying certain dlls to the local cache.
  8. Updated the Multicycle fitting engine to fix a crash when it was unable to find a G2 peak.
  9. Removed extra brackets from titles of picture plots showing data from Life Technologies Tali.
  10. Improved the appearance of resized pictures.
  11. Fixed a crash that occurred when changing the batch processing options, while the batch processing actions window was open but collapsed.
  12. Changed the default value for the Tali options to load background images.
  13. Fixed the not remembering the gate selected for performing parameter math.
  14. Fixed a crash that could occur when displaying image plots, especially while loading a layout.
  15. Removed surrounding spaces on the Current User token.
Version 4.03.0011 - October 29, 2012

Enhancements

  1. Added support for Life Technologies Tali data.
  2. Improved speed of drawing color dot plots showing colors for many gates.
  3. Improved speed of showing keyword values in the data list.

Bug Fixes

  1. Fixed a problem that manifested on several people's computers, where the gate and quad outlines, and statistics in text boxes on plots, would not update after the gate or quad move was complete, until the user clicked outside the plot.
  2. In 4.02.0015, the Page Visibility feature did not work unless you opened and closed the formatting of the page tab - this was fixed.
  3. Fixed a problem in using the keyword in plot titles.
  4. Eliminated a crash that occured on startup if someone had copied a file from Windows Explorer into the clipboard and then deleted the file.
  5. Updated the Tali data reader to allow a csv file name of the same name as the folder.
  6. Fixed a problem with the Tali data reader that caused all fields of view to be drawn on top of each other if the background image was not loaded.
  7. Fixed a crash that occured on startup if the scrollable area of the layout was set to a particular ratio of the total window size.
Version 4.02.0015 - September 8, 2012

Enhancements

  1. Updated Import Diva Facsdiva Add-on to better comply with Diva 7.
  2. If the setting for allowing Diva numeric file names is turned on, the Import Diva Add-on now allows those files to be used when importing the experiment.
  3. Added the ability to add fractional color levels for contour, density and heatmaps.
  4. Added a checkbox for forcing directory creation in batch processing actions.
  5. Improved speed of loading layouts and rendering plots.
  6. New batch processing report functionality for specific clinical customers.

Bug Fixes

  1. Resolved an issue introduced in 4.01.0026, which caused the data file names in plots to be expanded to their full UNC names - this meant that they didn't match what was in the Data List, and batch processing did not work as a result.
  2. Fixed a decimal separator problem when using the "," as the decimal separator, which affected custom tokens and custom datas.
  3. Improved alphabetical ordering when loading files into the data list, to match what is displayed in Windows Explorer.
  4. Fixed the fitting on Multicycle plots when the data was magnified.
  5. Fixed a crash that happened in some cases if you changed density plot color levels to a threshhold value that was too low.
  6. Previously, if the settings on a color dot plot were set to show new gates, but there were no gates on the layout yet, then if you created the first gate on a different plot, the original plot would not be redrawn with the new gate color.
  7. Fixed a problem with histogram subtraction in layouts imported from version 3.
  8. Improved error handling when loading picture plots with bad data.
  9. Corrected a crash that occured when formatting histograms with multiple overlays and markers, if during the format the person deleted an overlay or deleted a marker.
  10. Resolved a problem with expression handling inside formulas inside custom data cells, which occured when loading the layout or editing the cell, if the formula did not evaulate to a valid number.
Version 4.01.0029 - June 14, 2012

Enhancements

  1. Improved speed of displaying plots and changing pages.
  2. Improved speed of loading layouts.
  3. Added custom functionality for specific clinical customers.
Version 4.01.0027 - May 24, 2012

Enhancements

  1. Improved speed when loading layouts from network drives.
  2. Improved speed of deleting lists of custom tokens.
  3. Improved speed of opening the Layout History window for layouts with a lengthy history.

Bug Fixes

  1. Fixed a bug in batch processing which could cause the layout to freeze after completing the batch.
  2. Fixed a problem that caused certain old version 3 layouts to be very large when resaved in version 4.
Version 4.01.0026 - April 12, 2012

Enhancements

  1. Internet dongle enhancements.
Version 4.01.0025 - April 2, 2012

Enhancements

  1. Added support for the ImageJ data format.
  2. Added support for ICE format version 1.1.
  3. Improved stability and speed of internet dongle and site license servers.
  4. Improved the speed of dragging and dropping plots.
  5. Added new functions RemoveText and ReplaceText in custom tokens.

Bug Fixes

  1. Fixed a problem that prevented people with network licenses from adding new users.
  2. Fixed a problem with clinical launch files using layout map IDs.
  3. In cases where custom tokens contained other custom tokens, deleting them would sometimes cause the program to hang.
  4. Renaming custom tokens in some cases would cause a crash.
  5. Improved the handling of Z-order when copying a group of plots to the clipboard and pasting.
  6. Reenabled right-click->Delete on batch actions.
  7. Fixed a problem with saving the "below zero" value of a biexponential formula - previously, if the value was saved as 0 and the layout was saved, it was sometimes reset to 200.
  8. Resolved a crash that happened when deleting a linked gate.
  9. Reenabled deleting non-admin users in the Internet Dongle license.
  10. Improved the speed of setting up the Email to Notify in the security configuration window and fixed a crash that occured on invalid entries.
  11. Fixed a crash that happened in very large layouts when deleting a gate that was visible or used on many other plots.
  12. Improved the messages shown when building histograms for automatic compensation.
  13. Fixed undoing assigning compensations to a plot through dragging a compensation.
  14. Eliminated problems that happened when undoing signing a layout.
  15. Fixed a Windows resource problem that occured when using the navigator windows in very large layouts.
  16. Sped up typing in the Alerts windows.
  17. Fixed a crash that happened when double-clicking on a layout icon on the desktop, if that same layout was already open in FCS Express and was in the process of being closed.
  18. Allowed entering values lower than 90 for the JPG quality setting.
  19. Fixed a problem that caused files in the data list to be loaded in a non-alphabetical order.
  20. Prevented a crash when moving linked gates in a Gate View.
  21. Forced tokens to update to empty values when the data list is cleared, and the plots that the tokens are pointing to become empty.
  22. Prevented parameter math from running on embedded data files, when the option is set to run when files are loaded.
  23. Fixed the file open dialogs to properly display Windows folder shortcuts.
  24. Previously, outliers were not always drawn correctly for each outlier in contour plots where the palette is set to use logarithmic levels.
  25. Fixed a problem that caused Multicycle plots to perform an incorrect fit if the data was magnified.
  26. Resolved a memory leak that occured when copying plots to the clipboard.
Version 4.01.0018 - January 13, 2012

Enhancements

  1. Sped up plot display when adjusting manual compensation.
  2. Added copy protection information (countercode, dongle, internet dongle) to the About window.

Bug Fixes

  1. Fixed the problem that caused layouts saved or published from inside a batch processing report to lose plots on the 2nd pages.
  2. Fixed the duplication of keywords that occured on certain layouts after publishing a layout inside a batch process report.
  3. Fixed setting permissions on a published layout inside a batch processing report.
  4. Resolved a crash that could occur in the standalone license when the computer was waking up from being asleep.
  5. Restored the ability for network users to double-click on layouts and open them immediately.
  6. Fixed the display in the About window that caused standalone licenses to appear to be network.
  7. Resolved display problems in the automatic compensation setup messages.
  8. Fixed a crash that could occur when dragging and dropping a compensation matrix in a text box, if there was already other text in the text box.
Version 4.01.0015 - December 24, 2011

Known Limitations

  1. Network users will lose the ability to double-click on layout files, and automatically open them in FCS Express. Instead, you will need to first open the program, then open the layout. This will be resolved shortly in an upcoming release.
  2. If you are publishing the layout inside an XML in batch processing, then if there are hidden pages at the beginning of the layout, the plots on subsequent pages may not appear in the layout. This will be resolved shortly in an upcoming release.
  3. If you are using the security module with an ElevateDB database, the upgrade will not be backward-compatible. Please contact us for assistance.
  4. Internet dongle users are required to upgrade.

Enhancements

  1. Revamped and improved compensation infrastructure. There is a new dockable window with compensations, and an improved method of setting up automatic compensation.
  2. Improved management for the security system and internet dongle users, including the ability to log people out remotely, and to set up notifications for login failures or other events.
  3. Improved memory usage for picture plots.
  4. Improvements to the Proliferation fitting algorithm.
  5. Improvements to the contour plot outliers.
  6. Added options for gate text box placement.
  7. UI Enhancements: new buttons for zooming, and fewer clicks required to log out and change preferences.
  8. Implemented Ctrl + mouse wheel for zooming.
  9. Additions to the manual for Electronic Signatures
  10. Added options for changing the Edit/Selection colors of plots
  11. Improved speed of editing Users and Groups in the security module.
  12. Introduced a caching mechanism, to improve the speed of starting up FCS Express when running from a network drive.

Bug Fixes

  1. Fixed a problem that led to statistics not updating immediately if a plot had percentile quadrants, and the gate that the plot was gated on was modified, or the data or compnesation was modified, and the plot was not showing on screen.
  2. Resolved a crash that could happen if bad information was on the clipboard.
  3. Previously, dragging and dropping text boxes into plots did not work.
  4. Resolved crashes when importing certain CFlow files.
  5. Eliminated a crash that sometimes happened if the monitor turned off while FCS Express was running.
  6. Fixed a problem that led to a picture being specified as having 0 width in an XML export
  7. Fixed a potential hang after exporting to Excel.
  8. Fixed an occasional crash that happened when moving gates in plots with biexponential axes.
  9. Other stability improvements
Version 4.00.0057 - October 18, 2011

Enhancements

  1. Added the ability to read Nexcelom data files.
Version 4.00.0054 - July 18, 2011

Fixes

  1. Fixed a problem introduced in 4.00.0053 where the database repositories could not be installed.
  2. Fixed a problem where plots based on custom data using tokenized strings were not always updated when the layout was loaded.
Version 4.00.0053 - June 28, 2011

Enhancements

  1. Added the ability to read data in the ICE and ACS standard.
  2. Improved memory management of image data - loading image data sets is no longer limited to the amount of available RAM.
  3. Added SSMD and Z-Prime
  4. Now supports having more than one config file in the program settings folder - you will be prompted for which configuration to use.
  5. PDF version 1.6 is now supported in PDF batch reports.

Fixes

  1. Fixed a bug where quad stats did not immediately update visually when Pause between iterations was turned on in Batch Processing.
  2. Improved handling of scatter parameters from iCyt and InFlux data files so that they are displayed in linear by default.
  3. Renamed references to Isocyte from Molecular Devices to be ImageExpress Velos.
  4. Improved how the gate color precendence is calculated in dot plots.
  5. Previously, when adding an overlay to a histogram, the compensation was not automatically applied.
  6. Fixed reading in the parameter name keywords from the second data set of Beckman Coulter data files.
  7. Fixed a crash in drawing gates if the gate was too small.
  8. Fixed problems with Multicycle and CellProfiler data when used with Windows regional settings where the decimal separator is not '.'
  9. Enabled resizing columns in data grids.
  10. Enabled changing plot types for plots imported in version 3 layouts.
  11. Fixed a problem in exporting data files more than once.
  12. Uninstall now removes the program settings folder where possible.
  13. Improved the way range selection is calculated in Cell Number gates on data grids.
  14. Improved the appearance of options dialogs and wizards if Windows is set to use large fonts.
  15. Resolved a crash that happened when you started up a network version but did not have proper permissions on the network share.
  16. Previously, the % Positive statistic was not always recalculated on time.
  17. Improved the way the CFlow page options are handled during import.
  18. Ensured that histogram overlays from a CFlow import are all colored uniquely.
  19. Improved handling of dragging and dropping tokens onto plots.
  20. Fixed a crash that could happen if Office was not installed on Windows.
  21. Reduced flickering when navigating through tables inside text boxes.
  22. Fixed a bug that the parameter label settings were not always remembered in the axis label formatting category.
  23. The active state of batch processing actions is now remembered properly.
  24. Fixed the ability to magnify heat maps.
  25. Removed the program expiration date of Jan 1, 2012.
  26. Improved handling of Office .docx, .xlsx and .pptx files.
  27. Stopped requiring validation of batch actions that were deselected.
  28. Fixed a crash that occured if percentile quads were set to 100%.
  29. Fixed the legend color in bar plots.
  30. Improved the way axis labels are shown in surface plots.
  31. Other stability fixes.
Version 4.00.0023 - April 5, 2011

Enhancements

  1. Added several regression models: 5PL, 4PL, two-phase exponential fit, power series, Gaussian, Bolzmann, exponential association, sine wave, two-site binding, parabolic, cubic
  2. Added a node for plots with "No Data" in the Data Navigator.
  3. Enabled resizing columns in data grids.

Fixes

  1. Improved support for Multicycle in languages with a comma as the decimal separator.
  2. Changing parameter names in the header did not propagate changes to open plots.
  3. When dragging a data file into a histogram to create an overlay, an undo was not added to the undo system.
  4. Fixed a crash when you try to open the Axis formatting options on the ribbon for a bar chart.
  5. Improved stability when right-clicking in table cells and placeholders.
  6. Improved stability with Isocyte data when switching options between loading and not loading images.
  7. Fixed a crash that happened when closing a layout that had several dockable windows docked together.
  8. Previously, if a plot was using a parameter in a data file that no longer existed, it could trigger a crash.
  9. Fixed autosizing of statistics windows when loading version 3 layouts.
  10. Other fixes for loading layouts from version 3.
  11. Fixed a crash that could happen when displaying a log parameter with a non-automatic range.
  12. Fixed a crash when deleting an overlay that was used to normalize another one.
  13. Previously, dragging a file from the data list off and then back on to the list sometimes caused a crash.
  14. Fixed a problem when dragging a plot from one layout to another.
  15. Previously, FCS data files larger than 100 MB could not be written properly.
  16. Previously, if an Excel report specified a worksheet name which was blank or only had tokens in it, this could lead to an error.
  17. Fixed a problem with the Accuri import when trying to import histograms which had overlays, but not all overlays had data files.
  18. Fixed the page layout calculations in the Accuri import wizard.
  19. Other stability fixes.
Version 4.00.0010 - February 15, 2011

Enhancements

  1. Added the ability to take layouts saved in FCS Express 4 Plus and open them in FCS Express 4 Flow.
  2. Allowed Word batch processing export to use more than 60 merge fields.
  3. Added a warning dialog box when you save version 3 layouts.
  4. Updated the program icons to be distinct between Flow, Image and Plus.

Fixes

  1. Tokens for Multicycle statistics on a different page from the original plot did not always update in time.
  2. Multiselecting histogram overlays in the Overlays formatting category did not allow you to change the parameter.
  3. Previously, files without extensions were not being shown in the open data dialogs.
  4. Improved the way overlay lists are combined in the Quadrant and Overlays formatting categories when multiple plots are selected.
  5. In the Diva import, if a tube name had characters that were invalid for a filename (ie ;?/\) it was not displayed properly in the dialog that asks to select the acquisition filename.
  6. In the Diva import, if the files were located in a different folder than the XML file, then they were not found even if you selected them in a dialog.
  7. In the Accuri import, the axis options are now set to automatic by default.
  8. Fixed the background color property for picture plots.
  9. Fixed a crash that happened if you were in the middle of renaming a custom token and closed the layout.
  10. Fixed a crash in deleting multiple cells in a custom data grid.
  11. Fixed loading the background image for picture plots.
  12. Resolved a crash when displaying bar plots with a log axis.
  13. Fixed a problem with setting permissions for publishing using the advanced publisher.
  14. Fixed a crash that happened when the Select User dialog is open, and you double-click on a layout file in Windows Explorer.
  15. Prevented a crash in the Align feature when plots are grouped together.
  16. Fixed a problem changing the legend text in the Edit Preferences window.
  17. Prevented a crash when deleting position markers in Multicycle plots.
  18. Prevented a crash when you drag 2 or more files from Windows explorer into the Data List.
  19. Previously, changing the order of the batch actions was not always remembered.
  20. Improved the program behavior when you switch the number of monitors on your computer.
  21. Prevented a crash when you copy and paste a gate onto a plot from a plot showing different parameters.
  22. Stability fixes when loading in version 3 layouts.
  23. Other stability fixes.
Version 4.00.0000 - January 9, 2011
Initial release of FCS Express Version 4

Release Notes

Version 6.02.0003 - March 16, 2017

Bug Fixes

  1. Fixed a crash that occurred after copying quads from one plot to another, then linking them (53905)
  2. Double-clicking on downloaded layouts or published files will now be cause them to open in the Reader (53889)
Version 6.02.0002 - Feb 28, 2017 (Research Use Only)

Enhancements

  1. SPADE transformations are available (30241)
  2. Introduced Tiling Options to printing/saving to PDF/PPT during batch processing (42309)
  3. Introduced Tiling Options to saving to PPT (not during batch) (12232)
  4. Optimized database connections, and they are no longer dependent on ADO (30354)
  5. Can now drop a gate into marker endpoints, and a dialog will appear for selecting which statistic to link the endpoint to (30179)
  6. Batch Processing Runner window can now be docked (16911)
  7. In R transformation options, allow selecting how clustering results are represented (18822)
  8. In tSNE options, allow entering the number of iterations (40516)
  9. Improved the speed of working with compensation matrices (41472)
  10. Improved plotting speed with log parameters (41464)
  11. New Spreadsheet formulas available: RegressionPlotXtoY and RegressionPlotYtoX (41364)
  12. Improved speed of Spectral Plots (41218)
  13. Enabled the standard mac key combination for displaying options (Command + comma) (41165)
  14. Introduced an option for creating a File Identifier column when merging data files (41047)
  15. Improved speed when displaying the Format window for plots on Mac (40991)
  16. When dragging and dropping onto a Marker vertex, the marker vertex now becomes locked (40726)
  17. Multiple Duplicate and Next can now be created in a Custom Grid, regardless of the original position of the plots (42290)
  18. SOP Runner window can now be docked (42626)
  19. There is now a Mac-specific version of the manual (43227)

Bug Fixes

  1. Previously, if combination gates were used for coloring dots on more than one plot, then depending on the order in which the plots were drawn by FCS Express, the plots may not have been drawn to include all gate colors (42105)
  2. Data parameter tokens that are derived from a plot now correctly reflect the compensation on that plot (43474)
  3. Fixed a crash that could happen when there are specific kinds of tokens on the layout (eg “Current Username” token), and FCS Express’ connection to a database or internet dongle was disconnected (40684)
  4. On Mac, made sure that the “More Colors” selection in the color drop-down list displays a dialog (30390)
  5. Fixed a crash that occurred after adding an overlay to a heat map with a transformation (30327)
  6. Behavior for clicking with modifiers (Command key, Ctrl, Shift) on Mac was standardized (30127)
  7. Displaying contour plot outliers was made more reliable (41226, 30124)
  8. Fixed a crash that occurred after loading a layout, then replacing the data on a plot with a data file that has fewer parameters (30120)
  9. Prevented an error message that could be displayed while loading .ci files: “cumulativeData.txt has duplicate entries” (30070)
  10. Improved scaling of the Data Header window on high-DPI displays
  11. Fixed applying object insertion permissions when pasting an entire page (390)
  12. Fixed a crash that occurred after adding an overlay to plots with no visible overlays (40666)
  13. Improved stability of loading layouts with SRAW files (40624)
  14. Fixed a crash that could occur when dragging multiple gates inside the Gate View to a new parent gate (40711)
  15. Fixed a crash that occurred when inserting a token with an invalid cell value into a spreadsheet (41506)
  16. Prevented deleting from the transformations window when no items were present (41451)
  17. Fixed a crash when editing tokens inside the Options window, in the Plot Title options (41432)
  18. Improved display of text in Excel (Column Mode) action on Mac and high-DPI displays (41395)
  19. Improved display of the Multiple Duplicate and Next dialog on Mac and high-DPI displays (41369)
  20. Made sure that checkboxes appear on the popup menu for stat grids on Mac (41368)
  21. Fixed a crash that could occur during batch processing if the layout is showing more than one page at a time (41361)
  22. Enabled support for log in Spectrum plots (41316)
  23. Improved display in Batch Processing Runner window on Mac and high-DPI displays (41277)
  24. Fixed wording in error messages during R transformations (41265)
  25. Previously, gate text boxes could disappear after dragging a gate into a spreadsheet if 2 pages were displayed (41224)
  26. When editing existing transformations, if you change the template file, previously, the parameter list didn’t update (41214)
  27. Tokens inside spreadsheet cells derived directly from data files can now changes files in Next/Previous/Batch operations (41206, 42711)
  28. Previously, on Mac, Excel worksheets created during batch processing would not automatically open even if the checkbox was checked (41190)
  29. Prevented error message “Could not find parameter number; either the data is nil or the method is invalid” in cases when parameters are remembered by Name, and switching to a data file with fewer parameters (41089)
  30. Previously, when undoing replacing a histogram overlay, the wrong compensation might have been applied (40960)
  31. Fixed unlinking gates via the Gate Properties window (40930)
  32. Fixed a bug in tokens in the formula for Allow Editing gates, where the tokens would not track which plot they were on (17597)
  33. Improved spacing when rotating labels on the Y axis of plots (17735)
  34. Fixed crash report generation on Mac (40794)
  35. Previously, clicking in the top-left of a spreadsheet to select all cells cause FCS Express to hang (40823)
  36. Applying and un-applying transformations, that trigger the plots to change parameters, now including the parameter change when performing undoing (40845)
  37. Improved the way plots handle invalid parameters (40849)
  38. In certain cases, the Multiple Duplicate and Next dialog would previously not detect any available iterations (41371)
  39. Improved display in Axis options window on Mac (41394)
  40. Improved display of Histogram Specific options window on Mac (42131)
  41. Fixed a crash that occurred when showing the startup window after closing all layouts and logging out (42054)
  42. Improved speed when working in layouts with many nested snapshots (42060)
  43. Changed the keyboard shortcuts for Next/Previous data on Mac to be Alt+Left/Right instead of Command (42152)
  44. Fixed a crash that could occur after deleting markers in the format window, also led to markers being displayed in the wrong order and not being displayed on preview (42227, 18577, 42890)
  45. Previously, FCS Express could hang while importing certain ci files (42236)
  46. Improved display of axis labels on angles (42285)
  47. Fixed a crash when pasting tokens into the Title options (42307)
  48. Reduced the size of program dlls (42382)
  49. Previously, if you changed the Title options for several plots at the same time, and some were histograms and some 2d plots, and inserted a Current Gate token, the value would not be displayed on one of the types of plots (42409)
  50. Enabled a new report for the number of unique users logged in during a time period (42446)
  51. Fixed a crash that occurred after linking marker endpoints in a circular way (42473, 42548)
  52. Fixed a crash caused by editing parameter math items after reloading a layout (42477)
  53. Fixed the behavior of gates created on parameters that are no longer valid - previously, in some cases, all cells were being allowed (42478)
  54. Reenabled changing the order of files in the data list by dragging and dropping (42547)
  55. Previously, when gates were removed from TSNE plots, color dot plots continued to display their colors (42629)
  56. Fixed a crash when renaming a gate, if a heat map plot was open but not showing (42717)
  57. Previously, PDFs were automatically opened at the end of batch processing even if the checkbox for opening was turned off (42756)
  58. Compensation for new ci file formats is now being handled correctly (42807)
  59. Fixed a scenario where markers with linked endpoints were not always updated when the tokens updated, if there were more than one marker on the plot (42816)
  60. Previously, markers were sometimes shrunk to the axis after changing the legend options to always display solid boxes (42951)
  61. Improved the display of text in the Configuration wizard on Mac (43085)
  62. Previously, quad text boxes did not always update in real time when moving the quad centerpoint (43095)
  63. Improved the way data-specific gates and iterations interact with each other (43111)
  64. Previously, data-specific gates were lost when changing relatively location of data files on loading a layout (43114)
  65. Fixed a bug where extrakeywords.csv was not being read in correctly due Windows treating the csv delimiter differently in different locales (43126)
  66. Fixed a crash that could occur when dragging and dropping a plot into a spreadsheet cell to create a token, then selecting the overlay # as the statistic (43128)
  67. Fixed a crash on Mac that happened when editing the smoothing setting for multiple histogram overlays (43137)
  68. Improved the error message text when a purchase has not been approved (43162)
  69. Fixed a crash caused by applying Absolute to the color level selection when formatting a density plot (43207)
  70. Fixed the ImageJ Excel worksheet to work correctly on Mac (43326)
  71. Fixed a crash when viewing Cytoflex data files that have no events (43421)
  72. Improved stability on Mac (43625, 43634)
Version 6.01.0010 - December 8, 2016 (Research Use Only)

Bug Fixes

  1. Previously, when the Windows CHM help file was open beside a layout, searching in the CHM would sometimes lead to a crash
  2. Fixed a crash that occurred while sorting the Data List
  3. Fixed a crash that could occur on the Mac when selecting a file from the "Recent Places" list
  4. Fixed a crash that occurred when loading a layout saved with Data-Specific gates, if the original data files were missing and the data files were replaced during loading
  5. Fixed a problem that was introduced in 6.01.0009 that caused log-based color levels in density and contour plots to be displayed as linearly scaled if there were no negative numbers in the values
  6. Prevented a crash that occurred when editing compensation manually, if a blank was entered into the compensation value field
  7. Prevented a crash that could occur when using bar plots to plot spreadsheet data, if there was an invalid formula in the spreadsheet
  8. In version 6.01.0009, if you were updating from the previous version, the DNSEABridge executable was not replaced with the newer version - this has been fixed
Version 6.01.0009 - November 21, 2016 (Research Use Only)

Enhancements

  1. The BD Accuri importer can now import from .CI files as well as .C6
  2. Dragging a marker from one plot to another now creates a linked marker on the second plot
  3. Added a button for explicitly executing R scripts
  4. Parameter Math sequences from version 4 and 5 layouts are now imported into a new Parameter Math transformation.
  5. Improved storing transformation template files so they can be saved with relative paths
  6. Reduced the sensitivity of the mouse scroll wheel for MacOS
  7. Formatting quadrant text boxes now starts on the Contents category
  8. Improved handling of defaults for batch items in reports with field labels, where the field label has not been explicitly set
  9. Signing electronic signatures now requires entering the user name as well as password the first time in a session (subsequent signings only require the password)
  10. Errors the occur while performing R scripts and other transformations are now added to the layout's log (accessible on the status bar of the layout)
  11. Added an option for displaying histogram overlays in the legend using a box instead of a line
  12. The option for dithering colors on color dot plots has been changed to default to false
  13. Added coloring in compensation matrix tables in text boxes
  14. Dragging a marker onto an empty part of the page now inserts a text box and token, like it already did for gates
  15. Dragging data files onto a spreadsheet cell now pops up a dialog for selecting a stat or keyword token
  16. Added the ability to click on gate or quad vertices in a plot and select them even if they are "underneath" another gate
  17. Improved the usability of the crash dialog
  18. Improved the spacing between the labels and titles on vertical axes on plots

Bug Fixes

  1. Previously, when setting up quadrant authorizations, checking was not performed to prevent circular references
  2. Shortcuts on Mac now correctly use the Command key
  3. Fixed functionality for the printer icon on the quick access toolbar, and made sure that the print dialog was initialized correctly
  4. Prevented crash when performing parameter math using templates specifying non-existent files
  5. Fixed a crash triggered by data-specific gates when loaded in a layout where the data files could not be found
  6. Fixed a crash that occurred on heat maps when a gate had points with negative values
  7. Fixed copying and pasting of cells between spreadsheets on the layout
  8. Prevented a crash when attempted to calculate PCA using a gated subset that has no events
  9. Previously, permission rule formulas that included certain tokens did not always display correctly when the layout was reloaded
  10. Fixed a crash that occurred when adding a new compensation while the previous compensation matrix was being edited
  11. Changed the default folder for the auto-recovery file on MacOS to be in a standard folder
  12. Removed the incorrect % sign from density plot legends
  13. Fixed reordering items in the SOP list using drag and drop - previously the drop would put the item in an incorrect position in the list
  14. Fixed a crash that could occur in the Gate Navigator when the option to show plot nodes was turned off
  15. Improved the stability and error handling of the external application bridge
  16. Kinetics stats items now handle their gates being deleted gracefully without crashing
  17. Improved the way FCS Express interacts with MacOS in handling messages from the operating system
  18. Improved stability on MacOS
  19. Previously, spreadsheets changed their selected cell after zooming
  20. Disabled exporting data from spreadsheets using the ribbon Export button
  21. Eliminated an unnecessary Paste Special dialog when dragging cells from a data grid onto the page
  22. More UI fixes for high-resolution displays
  23. Spreadsheets no longer appear in the data list
  24. Prevented adding inappropriate statistic tokens for pie charts
  25. Improved Undo/Redo for changes inside a spreadsheet
  26. Simplified the behavior when dragging batch actions to reorder them
  27. Previously, FCS Express was unable to reopen layouts files referring to ICE data if the data had been moved on disk
  28. Removed transformations from the list of data that can be selected in the Export Data dialog - the transformations can be chosen separately in that dialog
  29. Made sure that compensation matrix tables in text boxes are displayed correctly even when the layout is set to remember parameters by name rather than number
  30. Simplified dragging vertices from one marker to another for creating links
  31. In the previous release, on MacOS a folder could not be selected after setting the data file type to FCS Plate Folder
  32. Fixed a crash that could occur when editing spreadsheet cells after merging cells
  33. Prevented changing plot types on plots that have no data loaded in order to avoid a crash
  34. Prevented sizing from being doubly applied when resizing pictures using the format window after using the Preview button
  35. Fixed a crash that could occur when dropping down the list of items in a token drop-down list
  36. Prevented spreadsheets from changing to a data file when using the Change on All buttons on the ribbon
  37. Ensured the pie charts update correctly if they are displaying spreadsheet data and the spreadsheet is on a page that's not currently visible
  38. Fixed a crash that could occur after renaming an SOP step without pressing Enter
  39. Previously, copying and pasting text with bullet items from PowerPoint into FCS Express caused a crash
  40. Fixed a crash that could occur in tSNE transformations if a parameter had no values
  41. Previously, using the configuration wizard with a network version to select a configuration file that was already saved on disk led to an error message
  42. Made tSNE calculations more robust
  43. Previously, on MacOS, selecting parameters on a plot in a list box that required horizontal scrolling was difficult to perform
  44. Fixed a crash that occurred in layouts showing Nexcelom data after opening an empty template and loading data into it
  45. Improved performance when opening the System tab in the Ribbon Backstage
  46. Fixed the usage of the default folders specified in the Options (previous it was sometimes using the most recent folder instead of the default)
  47. Previously, when plotting data from spreadsheets on dot plots, gates on those plots would include 100% of the data regardless of their position
  48. On MacOS, maximizing FCS Express now leaves room for the Dock
  49. After opening modal dialogs from the Ribbon Backstage, the title bar on the main window of the layout is now redrawn again properly
Version 6.00.0053 - September 24th, 2016 (Research Use Only)

Initial release of FCS Express 6